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Expr12388
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Worms grown under control conditions showed very miniscule fluorescence which was restricted to the spermatheca uterine valves. Furthermore, some minor fluorescence was observed in posterior and anterior parts of the worms. |
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Expr9433
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Adult Expression: stomato-intestinal muscle; anal depressor muscle; Reproductive System; vulval muscle; spermatheca uterine valve; body wall muscle; Nervous System; nerve ring; ventral nerve cord; dorsal nerve cord; lateral nerve cords; head neurons; neurons along body; tail neurons; Larval Expression: stomato-intestinal muscle; anal depressor muscle; Reproductive System; developing vulva; body wall muscle; Nervous System; nerve ring; ventral nerve cord; dorsal nerve cord; lateral nerve cords; head neurons; neurons along body; tail neurons; |
Sub-cellular localization within the body wall muscle: Dense bodies, Thick filaments and/or M-line, SR/ER |
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Expr12146
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Wild-type transgenic animals showed a constant expression of pxn-1 throughout their development, starting from the egg and continuing through to the adult stages. The expression patterns were associated with the ventral nerve cord (VNC), the dorsal nerve cord (DNC), body wall muscles, neurons, vulval muscles, uterine muscles, head neurons, intestine, and hypodermis. Moreover, the patterns were observed in excretory cells and in the spermathecal-uterine valve. |
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Reporter gene fusion type not specified. |
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Expr2894
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The longest construct, containing 10 kb of 5 upstream region from the first lin-3 exon, expresses lin-3::gfp in pharynx; spermathecal-uterine junction core cells and later in the spermatheca valve; pre-anchor (AC)/ventral uterine precursor (VU) cells and later in the anchor cell in the somatic gonad; vulF cells of the primary vulval lineage cells; and F, U and some of the B progeny cells in the male tail. This expression pattern was not affected by the different genetic backgrounds (dpy-20, pha-1 and unc-119) rescued by the corresponding co-injected rescue plasmids, implying that the gfp expression pattern is established by the lin-3 regulatory region. LIN-3 expression in different cells was temporally distinct as well. Expression in the pharynx was observed throughout post-embryonic stages. Spermathecal-uterine junction core cells, which later form the spermatheca valve, started expressing lin-3::gfp at the late L3 larval stage. |
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Expr13845
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The transcriptional reporter line exhibited strong MLCK-1 expression in the pharynx, anal sphincter, vulval cells, spermatheca, and sp-ut valve |
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CRISPR line COP1510 mlck-1::degron::mKate2 endogenously tagged with GFP strain, was generated by Nemametrix. |
Expr13846
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the CRISPR line showed MLCK-1 expression in the pharynx, uterus, spermatheca, and sp-ut valve. MLCK-1 is seen throughout the spermatheca, including the distal neck, bag, and valve cells. By mid L4, faint MLCK-1 expression is evident in the cells of the developing spermatheca. Expression levels peak in adulthood. During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. In adults, MLCK-1 is localized both apically and basally in the spermatheca. As expected, we observed thick bundles of actin at the outer, basal surface with a thinner actin network visible at the apical surface. MLCK-1 fluorescence intensity is brightest at the apical boundaries. However, some MLCK-1 is also localized to the basal cell surface, where it partially colocalizes with the contractile actomyosin bundles. |
During L4, MLCK-1 is localized to apical boundaries of the spermathecal cells, which face the lumen of the spermathecal bag. In adults, MLCK-1 is localized both apically and basally in the spermatheca. |
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Expr14341
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IFA-4::mKate2 showed expression in several of the same locations as exc-2: the excretory canals, the pharyngeal-intestinal valve, and the intestinal-rectal valve. IFA-4 is additionally located in the spermathecal-uterine valve and cells in the vulva, including the uterine muscles and two neurons in the tail. The IFA-4 protein was also found in the gut of dauer-stage but not well-fed worms, and appeared as well in the tips of the rays and neurons of the male tail. |
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Expr9279
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dhs-21::gfp fluorescence was especially prominent in the intestine, utse, gonadal sheath cells and sp-ut valve. First, diffused GFP fluorescence was detected as early as two-fold embryos, and the expression in intestine was observed at L1 through L3 stage. Then, the utse, gonadal sheath cells and sp-ut valve expression became obvious at L4. The spatial expression of intestine, utse and gonad continues up to 5-7 days. Male adults displayed a similar expression pattern, except for the utse, which is absent in males. |
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Supplemental Table S4. |
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Expr10841
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Supplemental Table S4. |
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Expr10853
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Expr15070
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mScarlet::PEZO-1, and PEZO-1::mScarlet were widely expressed from embryonic stages through adulthood. Notably, PEZO-1 is strongly expressed in several tubular tissues, including the pharyngeal-intestinal and spermathecal-uterine valves. Under higher magnification, we observed PEZO-1 on the plasma membranes of oocytes and embryonic cells during a variety of embryonic stages, suggesting PEZO-1 is a transmembrane protein. PEZO-1 is expressed in multiple reproductive tissues, including the germline, somatic oviduct, and spermatheca. Higher magnification imaging of the spermatheca revealed expression of PEZO-1 on sperm membranes as well. Live imaging and detailed analysis of PEZO-1 expression patterns during reproduction revealed that GFP::PEZO-1 is expressed in sheath cells, sperm, both spermathecal valves and the spermathecal bag cells. |
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Expr12840
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C. elegans nematodes that were genetically engineered to express GFP under the control of the zmp-2 promoter showed expression of zmp-2 earliest during embryogenesis in laid eggs. During the organogenesis/morphogenesis stage zmp-2 expression starts and multiple cells showed strong fluorescence in a complex manner in the early comma stage, the 1.5-fold stage, and the 3-fold stage. During larval development zmp-2 expression was concentrated in specific muscle and somatic gonad cells. In L4 larvae we observed strong signals in the developing spermatheca and weak signals in vulval muscles. In hermaphroditic adults the strongest expression was detectable in the two spermathecae and spermathecal-uterine valves. Anal cells as well as two cells in the head showed weaker expression. The head cells may represent motor neurons RMEV and RMED according to their position. Additionally, diffuse hypodermal GFP expression was observed in larvae shortly before molting events as well as in resting dauer larvae that start feeding and leaving their diapause. Within 1 h of accessing food, the animals usually exit the dauer stage and after further 8-9 h they molt to the L4 stage. Here, we determined strong hypodermal zmp-2 promoter regulated GFP expression of transgenic nematodes after 6.5-7 h after access to food shortly before molting process, providing further evidence for a role of ZMP-2 in ecdysis. |
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Strain COP1720 expressing spc-1::degron::mKate2 at the endogenous locus was generated by NemaMatrix (Eugene, OR, USA), characterized by genomic sequencing, and generously provided by the Zaidel-Bar Lab (Tel-Aviv University, Tel-Aviv, Israel). |
Expr13841
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SPC-1/α is prominently expressed in both the spermatheca and SP-UT valvebeginning in L4 and extending into adulthood. In adults, SPC-1/α is also faintly expressed in the sheath. |
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Expr13840
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Strong expression of UNC-70/β in the spermatheca and SP-UT valve begins early in spermathecal development during larval stage 4 and continues into adulthood. |
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Expr13842
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Beginning in L4, SMA-1/βH is seen in both the spermathecal bag and SP-UTvalve with the strongest expression in the 4 cells most proximal to the SP-UT valve. Interestingly, this expression pattern changes during spermathecal development, and by adulthood, SMA-1/βH becomes restricted to a ring connecting the spermathecal bag and SP-UT valve. |
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Expr10562
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A SIR-2.4::GFP translational fusion protein shows weak expression in most cell types. sir-2.4 is highly expressed in a subset of head and tail neurons beginning at early larval stage. High expression of sir-2.4 is also found in spermathecal-uterine valve cells beginning at L4 larval stage. |
The SIR-2.4::GFP fusion accumulates in the nucleus. |
