WormMine: AnatomyTerm WBbt:0006755

• Definition: sheath cell of phasmid sensillum
• Name: phasmid sheath cell
• Primary Identifier: WBbt:0006755

2 Children

Definition	Name	Synonym	Primary Identifier
Phasmid sheath	PHshR	lineage name: ABprpppapaa	WBbt:0004114
Phasmid sheath	PHshL	lineage name: ABplpppapaa	WBbt:0004116

4 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Single-cell RNA-Seq cell group 141_0 expressed in glia.	scVI 0.6.0	WBPaper00065841:141_0
	Top 300 transcripts enriched in ADE sheath cell, ADE socket cell, amphid sheath cell, amphid socket cell, cephalic sheath cell, CEP socket cell, IL sheath cell, IL socket cell, OL sheath cell, OL socket cell, PDE sheath cell, PDE socket cell, phasmid sheath cell, phasmid socket cell according to single cell RNAseq.	Top 300 enriched transcripts were determined by log2.ratio of the tpm in the cell type vs the tpm in the other cells * the log2 of the cell.type tpm.	WBPaper00061340:Glia
	Single-cell RNA-Seq cell group 42 expressed in: Unassigned hypodermis/gonadal sheath cells.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:42
	Single-cell RNA-Seq cell group 41 expressed in: Amphid and phasmid sheath.	CellRanger, DecontX, Monocle3, Louvain algorithm.	WBPaper00065623:41

21 Expression Patterns

• Primary Identifier: Expr11583
• Pattern: Paakg-5::GFP is expressed in the female gonad sheath cells, vulva epithelium and neurons, ventral cord neurons and excretory cell. It is also seen in the spermatheca and epithelial seam cells. In addition to the excretory cell, Paakg-5::GFP displays strong expression levels in the pharyngeal epithelia, neurons, some ring neurons and sensory neuron termini. In the tail, Paakg- 5::GFP signal mostly localizes to the pre-anal ganglion, rectum epithelium, intestinal-rectal valve and phasmid support cells. For a summary of Paakg-5::GFP see table S10.

• Primary Identifier: Expr11585
• Pattern: Head Paak-2::GFP expression is mostly seen in the excretory cell but is also observed in pharyngeal neurons, epithelial cells, a subset of ring neurons, amphid socket cells and head body wall muscles. Paak-2::GFP is also expressed in the posterior intestine, rectal gland and epithelial cells, and in phasmids. Paak-2::GFP is detected in vulval muscles. For a summary of Paak-2::GFP see table S10.

• Primary Identifier: Expr14140
• Pattern: ASI (dim), BAG, (I4), 3 more head neuron pairs, DD, VD, Amso, PHsh or Phso

• Primary Identifier: Expr9313
• Subcellular Localization: HMIT-1.3::EGFP was expressed at the lumenal membrane of intestinal cells. In addition, HMIT-1.3::EGFP was expressed in the cell body and the endfoot of glial cells.

• Primary Identifier: Expr9310
• Pattern: Phmit-1.3::egfp was expressed in the intestine and amphid sheath glial cells.

• Primary Identifier: Expr10193
• Pattern: Strong expression of bus-18 was observed in the rectal epithelial cells, but in addition wide-spread expression was observed in other epithelial cells. General expression in the hypodermis and vulva was observed, as well as in the labial support and arcade cells in the head, the excretory gland cell, the spermatheca, and the phasmid socket and sheath cells.

• Primary Identifier: Expr11586
• Pattern: Paakg-4::gfp is broadly expressed in C. elegans. Paakg-4::gfp is seen in tail sensory organs: phasmid sheath, socket cells and neurons, as well as in epithelial rectal cells, anal-depressor muscle, pre-anal ganglion rectal neurons, body wall muscles, posterior intestine and dorsal cord neuronal processes. Paakg-4::gfp is expressed in vulval, uterine muscles and ventral cord processes. Head expression mostly localizes to the ring ganglia plus 6 pharyngeal neurons. It is also seen in amphid sensory organs including sheath, socket cells and neurons. For a summary of Paakg-4::gfp see table S10.

• Primary Identifier: Expr9309
• Pattern: Phmit-1.2::egfp was expressed in the excretory canal cell, which is an osmotic regulatory organ that is functionally analogous to the kidney, and pairs of amphid and phasmid sheath glia.

• Primary Identifier: Expr9312
• Subcellular Localization: HMIT-1.2::EGFP was expressed in the cell body and the endfoot of glial cells. In the excretory canal cell, HMIT-1.2::EGFP was localized at the basal membrane of the distal canal and at the lumenal membrane of the proximal canal.

• Primary Identifier: Expr2401
• Pattern: In addition to hindgut cells, lin-48::gfp is expressed in the excretory duct cell, neuronal support cells of the phasmid and labial sensory structures and a small number of additional unidentified cells in the head. In the hindgut, these transgenes are expressed in U, F, K' and K cells. Male animals exhibit additional expression in the developing tail structures. lin-48::gfp expression is initiated in late embryogenesis and persists into adulthood.
• Subcellular Localization: The chimeric LIN-48::GFP protein is localized to the nuclei of expressing cells.

• Primary Identifier: Expr12998
• Pattern: PROS-1::EGFP is localized in the nuclei of the amphid sheath glia, the phasmid sheath glia and the excretory cell but not of the sensory neurons.

• Primary Identifier: Expr13751
• Pattern: R3::Deltapes- 10::gfp was expressed in phasmid sheath, hypodermal cells and rectal epithelial cells.

• Primary Identifier: Expr13753
• Pattern: R6::Deltapes-10::gfp was expressed in phasmid sheath and hypodermal cells.

• Primary Identifier: Expr12497
• Pattern: A kcc-3::gfp translational fusion is expressed in glial-like cells, including amphid sheath, cephalic sheath and phasmid sheath cells. The KCC-3::GFP signal was localized at the amphid sheath ending.

• Primary Identifier: Expr13070
• Pattern: BGNT-1.1 localises to the trans-golgi in the amphid and phasmid sheath cells.
• Subcellular Localization: BGNT-1.1 localises to the trans-golgi in the amphid and phasmid sheath cells.

• Primary Identifier: Expr12749
• Pattern: Expression of a fig-1 promoter::GFP reporter was detected exclusively within amphid and phasmid sheath glia and was first evident in late embryos, continuing throughout adulthood.

• Primary Identifier: Expr11525
• Pattern: F52E1.2 is exclusively expressed in the amphid and phasmid sheath (PHsh) cells. In the AMsh cells, some of the F52E1.2SP::YFP protein is transported to theAMsh cell endings, where it seems to be secreted into the AMsh lumen and localized around the cilia of the sensory neurons in wild-type.

• Primary Identifier: Expr12750
• Pattern: F16F9.3 is expressed only in amphid and phasmid sheath glia.

• Primary Identifier: Expr12751
• Pattern: F11C7.2 is expressed in amphid and phasmid sheath glia.

• Primary Identifier: Expr12752
• Pattern: F53F4.13 is expressed in amphid and phasmid sheath glia.

• Primary Identifier: Expr12753
• Pattern: T02B11.3 is expressed in amphid and phasmid sheath glia.

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
a structural (glial) cell which forms an specialized environment surrounding the sensory ending(s) of one or more neurons; sometimes accompanied by a more distal socket cell. In early development of the sensory nerves and of the nerve ring some sheath cells (cephalics and labials) may also provide a substrate for axon guidance	neuronal sheath cell	pocket cell	WBbt:0005811