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Reporter gene fusion type not specified. |
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Expr2608
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A 10 kb genomic promoter sequence is also expressed in vulval cells, which may not reflect the natural expression pattern of the ceh-2 gene, as that promoter extends into the next gene upstream. Reporter gene expression under the control of the ceh-2 promoter is detected shortly after formation of the pharynx primordium, and is strongest in elongated embryos and early larvae. 1.6 kb sequence upstream from the start codon drives expression only in the neurons (pTRB201); a fourth exon gfp fusion that includes 4.5 kb of upstream sequence and the large intron within the homeodomain is expressed also in e2 and m2 cells (pTRB202). |
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Expr2607
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Earliest expression with the antibody was seen in a small cluster in late-gastrulation embryos. From their position, these cells may be the precursors of the pharyngeal cells that express GFP in the L1 larva. ceh-2 expression was found restricted to eleven cells (fourteen nuclei) of five types in the anterior pharynx (corpus) of larvae and adults: the I3 neuron that lies embedded in the dorsal sector of the pharynx muscle; the pairs of NSM and M3 motoneurons in the left and right subventral sectors; the three m2 muscle cells, each possessing two nuclei resulting from cell fusion during development; and the three e2 epithelial cells with the anterior-most pharynx nuclei. |
Antibody staining is nuclear as expected for a transcription factor. |
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Reporter gene fusion type not specified. This information was extracted from published material (Archana Sharma-Oates, Andrew Mounsey and Ian A. Hope). |
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Expr626
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First detected prior to hatching, staying constant in most cells throughout the larval and adult stages. Expression seen predominantly in neurons, ventral nerve cord, DA, DB, VA, and VB class-set throughout the entire length of the cord and several pharyngeal neurons. Cytoplasmic expression is also seen in the dorsal cord, in the processes of DA3-7, DB1-7, PDB. Also in pair of unidentified neurons of lateral ganglia. In the male tail, expression is detected in the DVA neuron and the glial cells (Balpha.1/rd and Bgammapl/r)that form the socket for the spicule starting at the L4 stage. |
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