|
|
|
Expr13334
|
In hermaphrodites and males, selt-1.1 GFP expression was observed in neurons, epithelial and muscle cells of transgenic animals carrying both transcriptional (i.e. selt-1.2 promoter driving GFP expression) and translational (i.e. containing selt-1.2 promoter, exons and introns driving GFP expression) constructs. Crosses with the pan-neuronal marker rab-3 fused to the red fluorescent protein (RFP) in the nuclei confirmed that SELT-1.1 is expressed in all neurons of the nervous system. The expression of selt-1.1 in the ADL, ASH, ASI, ASJ, ASK, AWB amphid sensilla neurons was also confirmed by DiI staining. In the epithelia, selt-1.1 is expressed in the hypodermal, arcade, pharyngeal, vulval and rectal cells. selt-1.1 was not found to be expressed in the intestine or in the gonad. Muscle cells expressing selt-1.1 include the somatic muscle cells from head, neck and body wall as well as the non-striated pharyngeal muscles. SELT-1.1 expression was observed throughout development, from pre-bean embryonic stages to the adult stage. Embryos expressed GFP in most cells, also with a perinuclear localization. |
The translational Pselt-1.1::selt-1.1::gfp reporter revealed perinuclear localization, consistent with the ER localization previously reported for mammalian SELENOT. The ER localization of SELT-1.1 was confirmed by expressing into the QW1266[Pselt- 1.1::selt-1.1::gfp] the ER marker tram-1 fused to mcherry in muscle cells. |
