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Reporter gene fusion type not specified. |
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Expr1839
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During embryogenesis kal-1 expression is first detectable at about the 160- to 200-cell stage in a group of eight to ten neuroblast descendants of the AB blastomere. At no stage during embryogenesis were kal-1 driven reporters detectably expressed by epidermal cells. After elongation of the embryo is completed, the expressing cells have roughly reached the positions they will occupy in larval stages. During post-embryonic development, the expressing neurons remain largely the same, except for the recruitment of some sexually dimorphic neurons. Three groups of neurons express the kal-1 constructs post-embryonically. One group is located in the anterior ganglia. Here, about 15 neurons express the gene. They include some interneurons and some sensory neurons. A second group is located at mid body and is composed of the two canal associated neurons (CANs) and, in adult hermaphrodites, of the hermaphrodite specific neurons (HSN). The third group is located in the tail region, where three to six neurons express the construct. Among these are the PDB neuron and another interneuron, possibly PVW. Two male-specific neurons were also identified, the interneu on EF3 and one of the neurons of sensory ray 5. As during ventral enclosure, also during male tail formation, kal-1 is not expressed by epithelial cells but by neurons. |
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