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Expr11704
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Expression of the transcriptional fusion Ptop-1::gfp was not detected in early embryonic cells due to maternal germline silencing of the multi-copy transgenic gene. The GFP expression, however, was observed in most cells at the late embryonic stage and decreased with larval development. In the larval stages, GFP expression was predominantly present in the neuronal system, including sensory neurons (ILso, URX, RIC, IL1/IL2, AIY/AIM and RIG/RIF), motor neurons (VC4, VC5, HSN, PVD and PVM) of hermaphrodites and tail neurons (SPD and SPV/SPC) of males. The expression of the transcriptional fusion Ptop-1::gfp was strong in DTCs during the L3-L4 stage when gonad migration proceeds. |
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Expr12716
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Expr12717
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Data modified according to Shawn Lockery's expression pattern curations. |
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Expr296
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ASIf ASH SPDm/SPVm PVQ |
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Expr12263
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In contrast to hermaphrodites, lgc-35 is not expressed in any of the male enteric muscles, including the sphincter muscle, suggesting that it does not play a role in defecation in adult males. Like hermaphrodites, adult males expressed lgc-35 in acetylcholine motor neurons, the head mesodermal cell, and in the head interneurons AIY and AVD. In the male tail, lgc-35 is expressed in sensory rays 2, 3, 4, 5, and 9, the oblique muscle, the SPV neurons, and possibly the SPD neuron. |
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No detailed description on cellular expression pattern at hermaphrodites. |
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Expr1300
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LIN-29 accumulates in B cell progeny. In contrast to the LIN-29 accumulation pattern observed in wild-type hermaphrodites, LIN-29 is not detectable in the F, U, Y, or M blast cell nuclei, or in their progeny, in males. LIN-29 is detected in the progeny of B. LIN-29 is first detectable in B cell progeny during the L3 stage. Although all B.a and B.p progeny accumulate LIN-29, the LIN-29 accumulation signals appear weaker and transient in B.p progeny. The identification of these LIN-29-accumulating cells as B progeny is based on their size, shape, and relative position within the male tail and is further supported by laser microsurgery experiments and the examination of LIN-29-accumulation patterns in a mutant with defects in B cell specification. Elimination of the B cell by laser micro-surgery during the L1 stage reduced the number of LIN-29-accumulating nuclei in the male tail by 1015. LIN-29 appears to persist in B.a progeny in the adult stage. LIN-29 accumulates in the linker cell. The first male-specific LIN-29 accumulation is detected in the nucleus of the linker cell (LC) positioned at the tip of the growing end of the gonad. LIN-29 accumulates in the LC during the L3 stage, after the gonad arm has completed the 180 turn. LIN-29 remains detectable until the late L4 stage when its disappearance is presumably due to LC destruction. LIN-29 accumulates in the male tail seam. LIN-29 accumulates in the lateral body seam cell nuclei and in the SET nuclei in L4 stage wild-type males. LIN-29 accumulates in ventral cord nuclei. During the late L3 stage, five to seven nuclei that belong to the preanal ganglion accumulate LIN-29. Four preanal ganglion nuclei accumulates LIN-29 and were identified by position as the CA9, CP9, AS11, and VA11 neurons which are descended from P11.a. LIN-29 accumulation in the preanal ganglion cluster persists through adulthood. In late L4 stage and adult males, additional ventral cord nuclei anterior to the preanal ganglion accumulate LIN-29. The accumulation of LIN-29 in ventral cord neurons is not observed in hermaphrodites. Also in contrast to hermaphrodites, the ventral hypodermal nuclei positioned within the ventral cord do not contain detectable levels of LIN-29 in adult males. |
nuclei |
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Expr950
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Early in development, 1497pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. Early in development, pro-mab-3::gfp was expressed in head neurons of late embryos and L1 larvae of both sexes. Later in larval development, starting in L3, it was expressed in the lateral hypodermis (seam) in both sexes, and male-specifically in the nervous system, both in the head and tail, including the sensory ray neuroblasts R1-R9 in the tail. Initially there is some expression in the intestine of both sexes, but expression becomes male-specific by the adult stage, when vitellogenin transcription begins in hermaphrodites. |
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Expr15030
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In addition to male sex muscles, EGL-2::YFP is also expressed on the cell bodies, neural processes, and sensory endings of the male ray, post-cloacal, and spicule sensory neurons. the protractor muscles' EGL-2::YFP levels also increased between 24 and 48 h in wild-type males. |
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Data modified according to Shawn Lockery's expression pattern curations. |
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Expr293
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SPD/SPV, males only. |
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