WormMine: AnatomyTerm WBbt:0008587

• Definition: An apparatus for laying eggs of the hermaphrodite reproductive system, consists of the uterus, the uterine muscles, the vulva, the vulval muscles, and a local neuropil formed by the egg-laying neurons.
• Name: egg-laying apparatus
• Primary Identifier: WBbt:0008587

6 Children

Definition	Name	Synonym	Primary Identifier
female genital.	vulva		WBbt:0006748
muscle associated with hermaphrodite vulva.	vulval muscle		WBbt:0005821
The organ in which the eggs are developed and protected until laid.	uterus		WBbt:0006760
Neuron class of six motoneurons in the ventral cord, which innervate ventral body muscles and the vm2 muscles of the vulva.	VC neuron		WBbt:0005304
muscle lining of the uterine wall.	uterine muscle		WBbt:0005342
Neuron class of two motoneurons that innervate vulval muscles in the hermaphrodite.	HSN	hermaphrodite specific neuron	WBbt:0006830

0 Expression Clusters

6 Expression Patterns

• Primary Identifier: Expr13965
• Pattern: alkb-8 is expressed ubiquitously in embryos from approximately the 40-cell stage throughout the embryonic development. The expression continued in L1 larvae, although it was necessary to use longer exposure time for its visualization in accordance with the decreased expression observed in L1 larvae in the RT-qPCR experiment. The cytoplasmic expression of the transgene was strong in neurons, pharyngeal and body wall muscles, and other tissues such as somatic gonad and the egg-laying apparatus. We also observed diffuse expression in intestinal cells.

• Primary Identifier: Expr10997
• Pattern: Expression of nfi-1-GFP reporter constructs was observed in embryos, intestinal cells, body wall muscles, pharynx, egg-laying muscles, several head and tail neurons.

• Primary Identifier: Expr16049
• Pattern: nmgp-1 expresses mostly in sensory neurons and in the egg-laying apparatus of adult hermaphrodites. GFP expression driven by the putative nmgp-1 promoter was detected in several cells, primarily neurons. Within the head, we saw GFP expression in pharyngeal neurons, as many somas and processes within the metacorpus and the posterior bulb ventral ganglia were observed. In addition, some of the processes next to the bulb might correspond to CEP sheath glial cells. In the midbody, we saw labeling of cells within the egg-laying apparatus. Posteriorly, we observed cells in the tail ganglia and possibly phasmid neurons. Neuronal processes along dorsal and ventral cords were also labeled. In addition, to identify specific neurons, we used the NeuroPAL strain (OH15500) developed by Hobert's Lab (Yemini et al., 2021). This strain has a stereotyped fluorescent color map to identify all neurons. We injected it with the same plasmid for GFP expression under the nmgp-1 promoter. The following neurons were identified as expressing GFP: ALA, CEPD, IL1 (head neurons from the nerve ring), the sensory amphid neurons ASK, neurons from the anterior ventral nerve cord (VA6, VB7, DB5, AS5, VD6, DD3, DA4) and posterior ventral cord (VA11, VD11, AS10, DA7, DB7, CB11, VA11), neurons from the preanal ganglion (PVP, PVT, DD6, AS11, VA12, DA8, DA9) dorso-rectal ganglion (DVB, DVA, DVC) and lumbar ganglion (PVQ, PHC). The neurons identified include sensory neurons (amphid and mechanosensory), motor neurons and interneurons.

• Primary Identifier: Expr10531
• Pattern: atf-2::gfp expresses in the excretory duct cell, as well as other cells in the animal including the excretory pore cell and cells of the egg-laying and digestive systems (data not shown). atf-2::gfp expression initiates in the embryo and persists into larval and adult stages.

• Primary Identifier: Expr10594
• Pattern: Reporter transgene expression was observed in neurons in the head and tail; we also observed expression of irk-1 in the egg-laying system. Strong expression of Promirk-1::yfp was expressed in the HSN motor neurons of the egg-laying system, and faint expression of this reporter in vulval muscles, which are postsynaptic to the HSNs. Reporter transgene expression was observed in neurons in the head and tail; we also observed expression of irk-1 in the egg-laying system. We observed strong expression of Promirk-1::yfp in the HSN motor neurons of the egg-laying system, and faint expression of this reporter in vulval muscles, which are postsynaptic to the HSNs.
• Subcellular Localization: GFP::IRK-1 expression was observed on both the HSN soma and neurite. GFP::IRK-1 was not appreciably enriched at HSN presynaptic specializations. These data suggest that IRK-1 channels are uniformly expressed on the HSN plasma membrane and not restricted to a specific region of the cell.

• Primary Identifier: Expr16426
• Pattern: SER-7::GFP is expressed in the cells that normally express SER-7, including the egg-laying muscle cells and the VC neurons of the egg-laying system as well as a set of head neurons.

0 Life Stages

1 Parents

Definition	Name	Synonym	Primary Identifier
anatomical part of the body which is involved in sexual reproduction and constitutes the reproductive system.	sex organ	genitalia	WBbt:0008422