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Expr2276
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Neuronal expression. In the L1 stage cog-1::gfp is expressed in amphid neurons ADL(L/R), ASE(L/R), and ASJ(L/R). One additional pair of head neurons expresses cog-1::gfp in the L1. This pair is located between the excretory cell and the excretory pore, and is probably either AIA(L/R), SMBD(L/R), SIAD(L/R), or SIAV(L/R). In the tail, phasmid neurons PHB(L/R) express cog-1::gfp. The expression in amphid and phasmid neurons persists to the adult. Additional unidentified cells in the preanal ganglion express cog-1::gfp in late larvae and adults. Other cells in the tail region. The sphincter muscle (mu_sph) and phasmid sheath cells (PHshL, PHshR) express cog-1::gfp in all stages examined. Uterine expression. In the hermaphrodite gonad, cog-1::gfp is expressed exclusively in the dorsal uterine lineage. During the L3 stage, GFP is in the central four great-granddaughters of DU cells (DE4/DE5; DE, dorsal eight). The expression apparently persists in DE4/DE5 descendants, du, uv2, and uv3, until the adult stage, DE2 and DE7 lineages in the dorsal uterus also express cog-1::gfp starting from the early L4 stage. In the late L4, cog-1::gfp in this region is observed exclusively in sujc cells. Each core is surrounded by the spermathecaluterine valve and appears to form a plug which blocks the uterine lumen from the spermathecal lumen. None of the cog-1::gfp fusions, including the rescuing construct, was ever observed to express in any ventral uterine cells, including the anchor cell. Thus, the function of cog-1 in the connection is believed to be a consequence of abnormal gene regulation in the vulva, although a function in the dorsal uterine cells have not been ruled out. Vulval expression. In the vulva, cog-1::gfp is expressed from early L4 to the adult. Cells that express are vulC [P5.ppa(l/r), P7.pap(l/r)], vulD (P5.ppp, P7.paa), vulE(P6.pppl, P6.pppr, P6.paal, P6.paar), and vulF (P6.ppal, P6.ppar, P6.papl, P6.papr). The expression is considerably brighter in vulC and vulD than in vulE and vulF. Occasionally, expression in vulB and vulA was observed. Male expression. The expression of cog-1::gfp in the male was scored mostly in animals containing an extrachromosomal array containing the SmaI insertion. cog-1::gfp is expressed during proctodeal development. eL.aav and eR.aav express cog-1. In addition, proctodeal cells B.pap and B.pppa express cog-1::gfp. Additional expressing cells observed in occasional males include: rep, and P11.pp progeny. No gonadal cells in the male, including the linker cell, expressed cog-1::gfp in any stage of development. |
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