1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000024 | abu-1 | AC3.3 | Caenorhabditis elegans |
| Primary Identifier | Expr2207 | Remark | Type: mammalian cell transfection. Subcellular localization of C. elegans ABU-1 in mammalian cells, an experimental system with established markers for the various organelles and compartments. |
| Subcellular Localization | In transfected mammalian COS1 cells, metabolically-labeled ABU-1 tagged at its COOH terminus with the FLAG epitope remained associated with the cell pellet, from which it could be extracted only by detergent solubilization. Deletion of the predicted transmembrane domain led to secretion of the protein into the culture media. Immunostaining of the FLAG-tagged ABU-1expressing COS1 cells with anti-FLAG antibodies showed a diffuse reticular pattern that colocalized with the ER marker ribophorin I. These results suggest that ABU-1 encodes an integral membrane protein that is retained in the ER by its transmembrane domain. The secretion of transmembrane domain deleted ABU-1 suggests that the lumenal portion of the protein folds properly in mammalian cells and that the full-length ABU-1 is unlikely to be retained in the ER as a consequence of malfolding in mammalian cells. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00000024 | abu-1 | AC3.3 | Caenorhabditis elegans |
