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Expr14951
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In dauer animals, expression was observed in the intestine, five neurons, and faintly in the hypodermis. Intestinal expression starts from the posterior in uncommitted L2d and spreads throughout the entire intestine after dauer-commitment. Intestinal expression peaks in intensity during dauer, dims in starved and aged dauers, and disappears from the center of the intestine in post-dauer L4s. Neuronal expression is detectable in 58% of the dauer-committed L2d (n 1/4 15/26), and exists in all dauers (n 1/4 31). The neurons consist of one pair immediately anterior to the terminal bulb of the pharynx, with processes that end in the nerve ring, and one triplet approximately one cell body diameter posterior to the metacorpus, with processes that end in the nose. Based on their positions and morphologies, the best candidates for the neuron identities include: ADF, ADL, AFD, AIA, AIB, ASG, ASH, ASI, ASK, AWA, AWB, AWC, BAG, IL1, IL2, and OLQ. Using colocalization markers, we eliminated AIB, AWC, BAG, IL1, IL2, and OLQ as the ets-10-expressing neurons. The precise identities of the neurons remain unknown. In dauer animals, expression was observed in the intestine, five neurons, and faintly in the hypodermis. Intestinal expression starts from the posterior in uncommitted L2d and spreads throughout the entire intestine after dauer-commitment. Intestinal expression peaks in intensity during dauer, dims in starved and aged dauers, and disappears from the center of the intestine in post-dauer L4s. Neuronal expression is detectable in 58% of the dauer-committed L2d (n = 15/26), and exists in all dauers (n = 31). The neurons consist of one pair immediately anterior to the terminal bulb of the pharynx, with processes that end in the nerve ring, and one triplet approximately one cell body diameter posterior to the metacorpus, with processes that end in the nose. Based on their positions and morphologies, the best candidates for the neuron identities include: ADF, ADL, AFD, AIA, AIB, ASG, ASH, ASI, ASK, AWA, AWB, AWC, BAG, IL1, IL2, and OLQ. Using colocalization markers, we eliminated AIB, AWC, BAG, IL1, IL2, and OLQ as the ets-10-expressing neurons. The precise identities of the neurons remain unknown. |
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Reporter gene fusion type not specified. |
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Expr2590
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Animals expressing the daf-12::GFP construct display the highest levels of GFP throughout the procorpal region in the pharyngeal muscle (pm3) cells. GFP is also expressed throughout the metacorpus (pm4 cells) as well as the isthmus (pm5 cells) and portions of the terminal bulb (pm6). These transgenic animals appear to express GFP throughout the muscles of the pharynx and the pattern of expression is similar to uorescently labeled phalloidin which stains muscles in C. elegans. GFP orescence is detected in each developmental stage including dauer larvae. L2d animals, grown on pheromone plates, express GFP in the same cells, though it appeared brighter than animals of the other stages. |
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Expr13993
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Bright fluorescence was observed in the seam cells and glia socket cells of the anterior and posterior deirid neurons starting in the pre-dauer L2 (L2d) stage. Expression of dex-1p::gfp in the seam cells and deirid socket cells persisted throughout dauer. dex-1p::gfp expression was also observed in unidentified pharyngeal cells during all larval stages. |
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Expr14286
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We observed that phsp-4::GFP reporter was visibly induced in seam cells during two developmental stages -weakly in the late L4 stage and strongly in L2 stage animals on starved crowded plates- while it was undetectable in other larval stages. Closer examination suggested that hsp-4 expression is indeed induced in the posterior daughter cells, fated to differentiate into alae-secreting cells after the last asymmetric division. |
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Expr14949
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In dauer animals, expression occurs specifically in the hypodermis. Expression is undetectable under the dissecting scope in uncommitted L2d. Expression increases in the hypodermis 81-fold in dauer-committed L2d, 4,612-fold in pheromone-induced dauers, and 372-fold in starvation-induced dauers. The hypodermal expression remains but the intensity is significantly reduced in aged dauers and post-dauer L4s. |
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Expr3261
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The col-43::gfp fusion gene pC3894G was expressed in all hypodermal cells in the L2d stage. col-43 transcripts in the L2d stage were also confirmed by in situ hybridization. The L2d and dauer larvae develop to the adult worm through the L3 and L4 larval stages, respectively, after the environment shifts to nutrient-rich conditions. pC3894G was continuously expressed in L3; however, it was rarely expressed in L4 and adults recovered from L2d larvae. The expression of the col-43::gfp fusion gene pCTL3894G was not detected due to the signal peptide produced by this fusion gene. The COL-43::GFP fusion protein could be transported into the extracellular matrix and diffuse to an undetectable level. Two promoter deletion constructs, pC1345G and pC1292R, were expressed in the hypodermal cells in the L2d and the L3 stage recovered from the L2d stage. These promoter deletion constructs were continuously expressed in the dauer, the L4 and the adult recovered from stage L2. The additional promoter deletion construct, pC610G was also expressed in the hypodermal cells in the L2d and recovered L3 stage, but was rarely expressed in the L4 stage and adult recovered from the L2d stage. These results indicate that two upstream regions of col-43, within 0.6 kb and from 0.6 kb to 1.3 kb, were required for the expression and up-regulation in the dauer cycle of col-43 expressions, respectively. In the late adult stage the ectopic expressions of three promoter deletion constructs in the uterus and posterior intestinal cells were considered to be endogenous misexpressions of a series of pPD vectors. |
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Reporter gene fusion type not specified. |
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Expr2637
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A second promoter construct corresponding to the 1869 bp immediately upstream of the ATG start codon was also used to generate transgenic worms. This showed a similar pattern of expression in hypodermal cells, and was not expressed in the seam cells. Fluorescence was observed in late embryos and L2d predauer stages in the head hypodermal cells hyp 3, hyp 4, hyp 5, hyp 6, and hyp 7, also hyp 7 along the main body, and in tail hyp 8, hyp 9, hyp 10, and hyp 11. Lateral seam cells did not express cut-6. No expression of gfp was detected in any other stage, either larval or adult. M142.2/cut-6 is therefore expressed in the hypodermal cells. The pattern of spatial and temporal expression of M142.2/cut-6 in the daf-1 and daf-2 mutant backgrounds was identical to that found in the wild type N2 background, confirming that M142.2 was expressed in late embryos and L2d predauers. |
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Expr10798
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lin-31p::cfp and LIN-12::GFP are expressed in multipotent VPCs during larval (L)2 stage and before induction in the L3 stage. lin-31p::cfp expression remains high in VPCs of dauer larvae. LIN-12::GFP remains expressed in VPCs in dauer larvae. |
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Expr2636
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High levels of M142.2/cut-6 mRNA were detected during embryo and L2d predauer stages with little or no expression observed in the other developmental stages (L2, L3, L4, adult). |
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Expr13496
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Both 1st fate markers lag-2p::yfp and apx-1p::yfp were expressed as L2d larvae molt into dauer. The expression of 1st fate markers was extinguished in the VPCs of dauer larvae whereas the VPC identity markers were maintained. |
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Expr16450
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RT-PCR experiments showed that calu-1 mRNA is present in the dauer stage as well as at all reproductive larval and adult stages. |
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