1 Genes
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00016422 | noah-1 | C34G6.6 | Caenorhabditis elegans |
| Primary Identifier | Expr14713 | Subcellular Localization | NOAH-1 and NOAH-2 were distributed as a layer wrapping around the embryos with local differences, consistent with the notion that they are ECM components. Specifically, before the 2-fold stage, NOAH-1_mCH (int) and NOAH-2_GFP(int) were enriched above seam cells and in lines positioned like CeHDs at that stage. At later elongation stages, they displayed a fiber-like pattern in dorso-ventral (DV) cells, consistent with ZP proteins, which are often organized in filaments (Jovine et al., 2005). NOAH-2_GFP(int) displayed a dottier pattern and less obvious circumferential stripes at the 3-fold stage, perhaps because it was only partially functional. When combined, NOAH-1_mCH(int) and NOAH-2_GFP(int) showed colocalization, in particular at the circumferential stripes at late elongation stages. As the circumferential stripes were reminiscent of the actin bundles in the DV epidermal cells, we compared NOAH-1 distribution with that of epidermal actin visualized with a GFP-labeled actin-binding domain (referred to as ABD::GFP) (Gally et al., 2009). Deconvoluted confocal images and fluorescence profile measurement clearly indicated that NOAH-1 was apical to cortical actin, consistent with an extracellular localization. |
| WormBase Gene ID | Gene Name | Sequence Name | Organism |
|---|---|---|---|
| WBGene00016422 | noah-1 | C34G6.6 | Caenorhabditis elegans |
