WormMine: LifeStage WBls:0000102

• Definition: The whole period of embryogenesis in a nematode, from the formation of an egg until hatching.
• Primary Identifier: WBls:0000102
• Public Name: nematode embryo

0 Anatomy Terms

1 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	Any developmental stage in a nematode, including embryo, larva and adult stage.		Nematode life stage	WBls:0000101

6 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	C.briggsae proteins that showed significantly increased at L4 larva stage than in emrbyo.	Benjamin and Hochberg corrected p-value < 0.05.	WBPaper00051425:CB_L4_vs_embryo_upregulated
	C.briggsae proteins that showed significantly decreased at L1 larva stage than in emrbyo.	Benjamin and Hochberg corrected p-value < 0.05.	WBPaper00051425:CB_L1_vs_embryo_downregulated
	C.briggsae proteins that showed significantly decreased at L4 larva stage than in emrbyo.	Benjamin and Hochberg corrected p-value < 0.05.	WBPaper00051425:CB_L4_vs_embryo_downregulated
	C.briggsae proteins that showed significantly increased at L1 larva stage than in emrbyo.	Benjamin and Hochberg corrected p-value < 0.05.	WBPaper00051425:CB_L1_vs_embryo_upregulated
	Transcripts that showed significantly increased expression in ppa-daf-19(tu1035) and ppa-daf-19(tu1124) comparing to RS2333 wild type control samples.	Differentially expressed genes were identified by the program cuffdiff (version 2.2.1) from the comparison of both Ppa-daf-19 alleles with two wildtype control samples. The sets of up- and down-regulated genes (FDR corrected p-value <0.05).	WBPaper00055311:ppa-daf-19_upregulated
	Transcripts that showed significantly decreased expression in ppa-daf-19(tu1035) and ppa-daf-19(tu1124) comparing to RS2333 wild type control samples.	Differentially expressed genes were identified by the program cuffdiff (version 2.2.1) from the comparison of both Ppa-daf-19 alleles with two wildtype control samples. The sets of up- and down-regulated genes (FDR corrected p-value <0.05).	WBPaper00055311:ppa-daf-19_downregulated

2 Expression Patterns

• Primary Identifier: Expr11436
• Pattern: Cbr-puf-2 is expressed in the pharyngeal muscle 7. The GFP signal could only be detected during a brief window from the late fourfold embryo to the early second larval stage. Cbr-puf-2 reporter expression is also seen in four vulval muscle (vm) cells starting in L4. It is expressed in the anchor cell and vulval muscles, but not in the vulva precursor cells (VPCs) themselves.

• Primary Identifier: Expr12011
• Pattern: The earliest Ppa-obi-1 expression occurs in at least one cell in the pre-comma embryonic stage but soon disappears until weak expression reappears in the hyp7 and seam cells in J4 larvae. The most robust and diverse Ppa-obi-1 expression was found in mid-J4 hermaphrodites, which is consistent with real time quantitative PCR results indicating transcript levels in J2 and J3 larvae are less than in J4 larvae. In the mid-J4 stage, Ppa-obi-1 is strongly expressed in the major hypodermal syncytium and the lateral hypodermal cells that cover most of the body (hyp7 and seam cells) as well as the ventral tail Ppa-obi-1 is also strongly expressed in the P(5-7).p cell descendants that form the vulva lumen (vulC, D, E) during the mid-J4 stage until just prior to vulval eversion, as well as in the vulva muscles (likely vm1, 2). Most notably, Ppa-obi-1 expression was observed in the two bilaterally symmetrical specialized epithelial cells that envelope the chemosensory neurons, known as the amphid sheath cells. Ppa-obi-1p::gfp expression in the putative amphid sheath cells lie dorsal-lateral and posterior to the DiI labeled amphid neurons ASK, ADL, and ASI. Expression was also noticed in four unknown cell types anterior to the metacarpus (anterior pharyngeal bulb), which could be putative neuronal support cells for the inner labial or outer labial quadrant sensilla (IL or OLQ). Ppa-obi-1 is also expressed in putative duct and excretory cells on the ventral anterior side that form part of the excretory system.

2 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	A developemental life stage in a nematode that occurs from egg hatching until death.		postembryonic nematode	WBls:0000103
	A microfilaria life stage in which larvae are not sheathed by a membrane thought to be derived from the vitelline membrane.		unsheathed microfilaria	WBls:0000666

0 Preceded By

1 Sub Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The whole period of embryogenesis in the nematode Caenorhabditis elegans, from the formation of an egg until hatching.		embryo Ce	WBls:0000003