WormMine: LifeStage WBls:0000063

• Definition: At 20 Centigrade: 0-24 hours after L4-adult molt.
• Primary Identifier: WBls:0000063
• Public Name: newly molted young adult hermaphrodite Ce

0 Anatomy Terms

2 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage after an hermaphrodite animal is fully-developed and reaches maturity.		adult hermaphrodite Ce	WBls:0000057
	A C. elegans life stage that occurs during the adult life stage		C. elegans life stage occurring during adulthood	WBls:0000816

0 Expression Clusters

29 Expression Patterns

• Primary Identifier: Expr15065
• Pattern: In hermaphrodites, ENRI-3::GFP was detected in the maturing germline of L4 larvae, with its expression peaking during spermatogenesis and in mature sperm in young adult hermaphrodites. ENRI-3 localization was concentrated in distinctive perinuclear puncta around germline nuclei, and co-localizes with the P granule marker PGL-3. The strongest expression of ENRI-3 was detected in the germline cytoplasm of male animals. ENRI-3 could also be detected in embryos and early larval stages, but its expression was limited to the precursor cells of the germline (Z2 and Z3).
• Subcellular Localization: ENRI-3 localization was concentrated in distinctive perinuclear puncta around germline nuclei, and co-localizes with the P granule marker PGL-3.

• Primary Identifier: Expr14590
• Pattern: Embryonic expression of exc-7 was first observed at the bean stage. By reverse lineaging with use of SIMI-Biocell software, we confirm the identity of one of the expressing cells at this stage as the excretory canal cell. In L1 animals, broad expression in the head, ventral nerve cord (VNC), and tail was observed. In young adults, expression is notably observed in vulva cells. In the nervous system specifically, expression is observed in many neurons throughout the body, but unlike Drosophila Elav, exc-7::gfp it is not panneuronally expressed. We confirmed previously reported expression in cholinergic VNC MNs, but absence of GABAergic VNC MNs, consistent with previous reports (Fujita et al., 1999; Loria et al., 2003) and consistent with exc-7 functioning in cholinergic, but not GABAergic neurons to control alternative splicing (Norris et al., 2014). exc-7::gfp is also expressed in some non-neuronal cell types, including muscle and hypodermis, but not in the gut. A previous report showed that exc-7 is only transiently and weakly expressed in the excretory cell, which, based on exc-7's excretory mutant phenotype, has puzzled researchers (Fujita et al., 2003). We find that the gfp tagged exc-7 locus is strongly and continuously expressed in the excretory canal cell.

• Primary Identifier: Expr14555
• Pattern: hpx-2 is expressed in the hypodermis and pharynx in young-adult and dauer worms. In dauers, we observed weak expression of hpx-2::GFP in the distal bulb of the pharynx in nearly all animals. At higher magnification, the localization pattern was most consistent with expression in the gland cells.

• Primary Identifier: Expr13710
• Pattern: The cisd-1 (pnls27) animals showed GFP expression in the germline of L4 and young adults. The GFP expression was also observed in embryonic blastomeres, and the muscle and intestinal cells within larvae and young adults.

• Primary Identifier: Expr12532
• Pattern: RAD-23 expression was detected throughout the animal at the young adult (L4 + 1 d) stage, including neuronal cell bodies and processes, the vulva, the nerve cord, and throughout the head.
• Subcellular Localization: RAD-23 protein is expressed in the cell body and processes of a mechanosensory neuron.

• Primary Identifier: Expr16214
• Pattern: Pedem-3::GFP expression was detected in the pharynx, nervous system, body wall muscle (mosaic expression), coelomocytes, hindgut and tail structures, sensory neurons, and CAN neurons. In young adults the expression became apparent in vulva muscle and vulval epithelium, uterus, distal tip cells, and hermaphrodite specific neuron (HSN). Pedem-3::GFP expression in the gut was transiently activated in L1 and L2 larvae, then faded and reappeared in older animals (mosaic expression).

• Primary Identifier: Expr13901
• Pattern: Prib-1::gfp is broadly expressed in ectodermal and mesodermal cells during embryogenesis. A salient feature of the rib-1 expression pattern is that it is very dynamic in hypodermal cells during development. In embryogenesis, Prib-1::gfp is detected along the entire layer of hypodermoblasts that surrounds the gastrulating embryo at about 200 minutes after fertilization. By the early comma stage of embryogenesis, Prib-1::gfp is expressed at high levels in hypodermal cells of the elongating embryo, including hypodermal cells extending ventrally during ventral closure and in the two rows of dorsal hypodermal cells undergoing dorsal intercalation. Following these embryonic morphogenetic events, expression of Prib-1::gfp in the hypodermal cells of the body wall is no longer visible during larval and adult stages, except for seam cells undergoing fusion during larval development. Also, hypodermal cells of the developing vulva express Prib-1::gfp, at a low expression level in L3 larvae and at a stronger level in L4 larvae and just molted young adults, and vanishing in vulval cells in the adult. The nervous and digestive systems express Prib-1::gfp stably and continuously from embryogenesis throughout adulthood. Strong and sustained expression is seen in motorneurons, interneurons, sensory neurons (including AVM), neurons in the head and tail ganglia, with the GFP signal filling axons running along the ventral and dorsal nerve cords, commissures, and sublaterals. Expression in neurons of the ventral nerve cord and of the head ganglia is visible in 1.5-, 2-, and 3-fold embryos, and persists into adulthood. Strong expression of Prib-1::gfp is also observed in the pharynx from the 2-fold stage of embryogenesis onwards and remained strong in adults (procorpus, metacorpus, terminal bulb, grinder, and pharyngeal-intestinal valve). The anal depressor, the anal sphincter, the two enteric muscles, the spermathecae and the uterine muscles maintain expression in adults.

• Primary Identifier: Expr13937
• Pattern: Broad aak- 2::GFP expression was observed in the pharynx, gut, and nervous and reproduction systems of the young adult animals.

• Primary Identifier: Expr12540
• Subcellular Localization: AMPH-1/BIN1 is found at the nuclear rim in C. elegans seam and intestinal cells.

• Primary Identifier: Expr12539
• Subcellular Localization: In intestinal cells of young adult animals, endogenous ANC-1 accumulated at the nuclear rim and also decorated perinuclear structures.

• Primary Identifier: Expr16248
• Subcellular Localization: SET-13 and SET-25 were enriched in the nucleus. SET-13::GFP was expressed through eight-cell stage to late-embryonic stage, while SET-25::GFP was expressed in the whole embryonic stage and the germline in young adults.

• Primary Identifier: Expr13690
• Remark: AFP
• Pattern: SYGL-1 expression was found in the distal-most cells in the germline, also expressed in the proximal loop region and oocytes in young adult hermaphrodites. Mostly cytoplasmic.

• Primary Identifier: Expr13691
• Remark: AFP
• Pattern: LST-1 expression was found in the distal-most cells in the germline, also in the proximal loop region and oocytes in young adult hermaphrodites. Mostly cytoplasmic, enriched in peri-nuclear granules at the distal-most germ cells.

• Primary Identifier: Expr16423
• Pattern: We were able to reproduce the NHR-85::GFP peak in expression in vulval precursor cells from L4.0 to L4.9. We also observed the reported expression in hypodermal cells as well as in seam cells. Interestingly, we did not observe NHR-85::GFP expression in the L4 or adult germline.

• Primary Identifier: Expr16424
• Pattern: We found a similar pulse of NHR-23::mScarlet in L4.3 vulval precursor cells as that reported by Kinney et al.. nhr-23::mScarlet was also detectable in seam and hypodermal cells at this stage, epithelial cells that synthesize cuticular components. NHR-23::mScarlet was detected in pachytene nuclei in L4 animals and its zone of expression became restricted in young adult animals. This expression pattern is again similar to NHR-23::GFP::AID*::3xFLAG (Ragle et al. 2020). However, we also observed some interesting differences compared to NHR-23::GFP::AID*::3xFLAG. In our previous work, we observed that NHR::23::GFP became undetectable in ovulating adults (Ragle et al. 2020). In contrast, NHR-23::mScarlet appears to be diffusely expressed in young adult residual bodies and spermatocytes. This diffuse expression persists into ovulating adults, and appears restricted to the spermatheca. The NHR-23::mScarlet signal is specific to nhr-23::mScarlet::3xMyc animals as no diffuse expression in the germline is observed in wild-type animals. One can observe the diffuse expression pattern in L4.5 germlines several rows of cells after NHR-23::mScarlet::3xMyc localization is lost from nuclei.

• Primary Identifier: Expr13668
• Pattern: C08B6.4 GFP expression in the 3-fold stage of C. elegans was localized to hypodermal cells and also appeared to be secreted into the space between the hypodermal syncytium and the cuticle, suggesting that this putative chitinase might also play a role in cuticle formation in the L1 and/or the initial molt to the L2. In addition to hypodermis, C08B6.4 also was expressed in several anterior amphidial neurons in all larval stages and the young adult.

• Primary Identifier: Expr15509
• Pattern: Consistent with previous reports, HSP-17 was expressed in the young adult excretory cells. HSP-17 was not expressed in the hypodermis.

• Primary Identifier: Expr15510
• Pattern: Consistent with previous reports, ZK1128.7 was expressed in the young adult vulva muscles. ZK1128.7 was not expressed in the epidermis.

• Primary Identifier: Expr14301
• Pattern: TBP-1::GFP is expressed in gonad nuclei of a young adults.

• Primary Identifier: Expr16487
• Pattern: fkh-8::GFP fosmid expression at young adult stage is detected in all ciliated sensory neurons, as assessed by co-localization with the ift-20 core ciliome reporter. Three non-ciliated neurons, PVD, VC4 and VC5 show FKH-8 expression, while no expression is detected in non-neuronal tissues. C. elegans male nervous system contains additional ciliated sensory neurons, mostly in the tail, which also express FKH-8.During embryonic development, there is a similar overlap between FKH-8 and ift-20 reporters.

• Primary Identifier: Expr14296
• Pattern: To gain insight into the role of AP endonucleases a er embryogenesis, we measured the temporal change in the mRNA expression level of exo-3 or apn-1. At 0, 24 and 48 hours, when most N2 worms are in the egg stage, L1 stage and L4 stage, respectively, no di erence in mRNA expression level was found for both exo-3 and apn-1. At 60 hours, when most N2 worms are in the young adult stage, the exo-3 and apn-1 expression levels were approximately 13-fold and 2.3- fold higher than those at 0 hours, respectively. e expression level at 72 hours, when most N2 worms are in the gravid adult stage, was the same at 60 hours. These results suggest that AP endonucleases are required after embryogenesis, especially a er the L4 stage.

• Primary Identifier: Expr14965
• Pattern: We used sups-1 regulatory sequences to drive GFP-tagged Histone H2B. GFP::H2B was present in epidermal nuclei in late embryos, larvae and young adults, but we could not detect GFP::H2B in other tissues, including thespermatheca.

• Primary Identifier: Expr13404
• Pattern: cdc-25.3 mRNA is highly expressed in N2 hermaphrodites at the young-adult stage, when oogenesis actively occurs, and in feminized fem-1(lf) hermaphrodites, but not in adult males or in masculinized fem-3(gf) hermaphrodites. These results indicate that cdc-25.3 is preferentially expressed during oogenesis.

• Primary Identifier: Expr13252
• Pattern: HSF-1::GFP is localized to the nuclei of all major somatic tissues in L2 and young adult animals.

• Primary Identifier: Expr13284
• Pattern: mRNA levels of both cyb-1 and cyb-3 were high in young-adult and adult N2 hermaphrodites as well as in feminized fem-1(hc17lf) adult hermaphrodites. By contrast, cyb-1 mRNA, but not cyb-3 mRNA, was highly expressed in the L4 stage N2 hermaphrodites, adult N2 males, and masculinized fem-3(q20gf) adult hermaphrodites, in all of which spermatogenesis proceed specifically and robustly. On the other hand, mRNA levels of both cyb-1 and cyb-3 were negligible in germline proliferation-defective glp-1(q224lf) adult hermaphrodites. These results indicate that cyb-1 is the major B-type cyclin robustly expressed during spermatogenesis.

• Primary Identifier: Expr13283
• Pattern: mRNA levels of both cyb-1 and cyb-3 were high in young-adult and adult N2 hermaphrodites as well as in feminized fem-1(hc17lf) adult hermaphrodites. By contrast, cyb-1 mRNA, but not cyb-3 mRNA, was highly expressed in the L4 stage N2 hermaphrodites, adult N2 males, and masculinized fem-3(q20gf) adult hermaphrodites, in all of which spermatogenesis proceed specifically and robustly. On the other hand, mRNA levels of both cyb-1 and cyb-3 were negligible in germline proliferation-defective glp-1(q224lf) adult hermaphrodites. These results indicate that cyb-1 is the major B-type cyclin robustly expressed during spermatogenesis.

• Primary Identifier: Expr14297
• Pattern: To gain insight into the role of AP endonucleases a er embryogenesis, we measured the temporal change in the mRNA expression level of exo-3 or apn-1. At 0, 24 and 48 hours, when most N2 worms are in the egg stage, L1 stage and L4 stage, respectively, no di erence in mRNA expression level was found for both exo-3 and apn-1. At 60 hours, when most N2 worms are in the young adult stage, the exo-3 and apn-1 expression levels were approximately 13-fold and 2.3- fold higher than those at 0 hours, respectively. e expression level at 72 hours, when most N2 worms are in the gravid adult stage, was the same at 60 hours. These results suggest that AP endonucleases are required after embryogenesis, especially a er the L4 stage.

• Primary Identifier: Expr16169
• Pattern: Our results indicate that hsf-1 expression steadily declines during development as hsf-1 mRNA transcripts were most abundant during embryonic development (in eggs), less in L2, even less in L4 larvae, while the least amount of hsf-1 mRNA was detected in young adults.

• Primary Identifier: Expr16450
• Pattern: RT-PCR experiments showed that calu-1 mRNA is present in the dauer stage as well as at all reproductive larval and adult stages.

0 Followed By

0 Preceded By

0 Sub Stages