WormMine: LifeStage WBls:0000108

• Definition: The third stage larva of nematodes.
• Primary Identifier: WBls:0000108
• Public Name: L3 larval stage

0 Anatomy Terms

1 Contained In

Remark	Definition	Other Name	Public Name	Primary Identifier
	The stage that begins after hatching and ends when the nematode becomes adult.		nematode larval stage	WBls:0000105

4 Expression Clusters

Regulated By Treatment	Description	Algorithm	Primary Identifier
	Transcripts that showed significantly increased expression in Cbr-htz-1(gu167) comparing to in AF16.	DESeq2, FDR < 0.05.	WBPaper00059178:Cbr-htz-1(gu167)_upregulated
	Transcripts that showed significantly decreased expression in Cbr-spr-4(gu163) comparing to in AF16.	DESeq2, FDR < 0.05.	WBPaper00059178:Cbr-spr-4(gu163)_downregulated
	Transcripts that showed significantly increased expression in Cbr-spr-4(gu163) comparing to in AF16.	DESeq2, FDR < 0.05.	WBPaper00059178:Cbr-spr-4(gu163)_upregulated
	Transcripts that showed significantly decreased expression in Cbr-htz-1(gu167) comparing to in AF16.	DESeq2, FDR < 0.05.	WBPaper00059178:Cbr-htz-1(gu167)_downregulated

2 Expression Patterns

• Primary Identifier: Expr11516
• Pattern: The developmental profiles of Cbr-puf-2 and Cbr-puf-1.2 mRNA levels are qualitatively similar and are typical of germ line-expressed genes: low expression from embryo to L2 stages, slightly increasing expression at L3 and L4, and peak levels in adults. However, Cbr-puf-2 is over 100-fold more abundant than Cbr-puf-1.2.

• Primary Identifier: Expr12798
• Pattern: tat-3 reporter signal first appears in embryos in the developing pharynx. In the fully formed alimentary system, very strong GFP fluorescence is observed in the muscle, marginal and buccal epithelial cells of the pharynx, the pharyngeal-intestinal valve and, with lesser intensity, the rectal epithelial cells. Seam cells display very strong fluorescence as soon as this lineage becomes established during embryonic development. In adults, moderate to weak fluorescence seems to arise from the XXX cells, some unidentified cells in the head and tail regions and the hypodermis. In the reproductive system, tat-3 reporter expression begins in the distal tip cells (DTC) in L1 and in the anchor cell (AC) in early L3. GFP signal is later visible in the dividing progeny of the vulval precursor cells (VPCs). In late L4, the anchor cell fuses with the uterine seam cell (utse), which does not express the reporter. The vulval cells continue exhibiting moderate fluorescence into the adulthood.

1 Followed By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The fourth stage larva of nematodes.		L4 larval stage	WBls:0000109

1 Preceded By

Remark	Definition	Other Name	Public Name	Primary Identifier
	The second stage larva of nematodes.		L2 larval stage	WBls:0000107

3 Sub Stages

Remark	Definition	Other Name	Public Name	Primary Identifier
	The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch.		L3 larva Ce	WBls:0000035
	The third stage larva.		Brugia L3	WBls:0000081
	Strongyloides infective stage L3 larval stage. This stage can develop as a part of a free-living life-cycle, or from a parasitic, parthenogenic female stage.		Strongyloides infective L3	WBls:0000680