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Expr1790
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daf-9 was strikingly expressed in a ventral pair of bilateral neurons identified as the IL1Vs or URAVs in the anterior ganglia. Neuronal expression first appeared shortly before hatch, increased greatly during larval development, and perdured in reproductive and postreproductive adults. By mid-L2, DAF-9 also appeared in the cytoplasm of the hypodermis, the syncitial epidermis surrounding the worm, but was absent from midline epidermal seam cells. Levels peaked around the L2 molt and diminished during L4. In some cases, transient expression was seen in L3 vulval blast cells. daf-9 was also expressed within the hermaphrodite spermatheca starting in late L4 larvae, and continuing even in old adults. In some extrachromosomal lines, sporadic weak expression was seen in the excretory cell, a few unidentified head neurons, and vulval muscles. In males, daf-9 was expressed in IL1V/URAVs and hypodermis but not somatic gonad. In dauer larvae, daf-9 was strongly expressed in IL1V/URAV and specifically extended into axonal but not dendritic processes. No hypodermal expression was evident in dauers. In post-dauer stages, daf-9 was expressed in a pattern similar to reproductively growing animals, except expression was absent in the hypodermis. |
DAF-9 was perinuclear, consistent with residence in endoplasmic reticulum. |
