| Algorithm | The significance (P 0.0001) of the relative age (time) was used to determine if a gene was differentially expressed between the three age (time) groups. The effect of this factor explaining gene expression differences was used to determine if the expression went up or down during the two age/time periods (t1 - t2 and t2 -t3). Authors used a permutation approach to determine the thresholds for the different mapping strategies. For each of the used models for eQTL mapping, authors used 23,000 permutations. For each permutation, authors randomly picked a spot; each spot could only be picked once. The gene expression and relative lifespan values were than randomly distributed over the RILs (and time points) and used for mapping. In this way, authors obtained a threshold for each of the explaining factors. For the single time points, authors used a FDR of 0.01 to adjust for multiple testing. The genome-wide threshold for this FDR is -log10 P = 3.8 for each of the three time points. For the combined models (t1 to t2 and t2 to t3), authors used a genome-wide threshold of -log10 P = 4, which resembles an FDR of 0.006, 0.001, and 0.006 for marker, age, and the interaction between marker and age, respectively. To determine the threshold for the single gene examples, authors used 1000 permutations as in the genome-wide threshold. The difference is that they use the gene under study in all of the permutations. The P-values for the gene specific thresholds were determined at FDR = 0.05. | Description | Expression Pattern Group D, enriched for genes involved in catabolic processes. |
| Primary Identifier | WBPaper00036286:Pattern_D |
| Remark | Definition | Other Name | Public Name | Primary Identifier |
|---|---|---|---|---|
| The third stage larva. At 25 Centigrade, it ranges 32.5-40 hours after fertilization, 18.5-26 hours after hatch. | L3 larva Ce | WBls:0000035 | ||
| At 20 Centigrade: 7-10 days after L4-adult molt. 10-13 days after first cleavage. | 7-10 days post-L4 adult hermaphrodite Ce | WBls:0000070 | ||
| At 20 Centigrade: 48-72 hours after L4-adult molt. 5-6 days after first cleavage. | 2-days post-L4 adult hermaphrodite Ce | WBls:0000065 |
