WormMine: RNAi WBRNAi00107863

• WormBase ID: WBRNAi00107863
• Genotype: DYN-1::GFP
• Phenotype Remark: "As described previously, DYN-1-GFP foci localize across the cortex before the onset of polarity establishment in wild-type embryos. The foci become enriched in the anterior and are maintained through cytokinesis (Figure 2C, control; Nakayama et_al, 2009). In arx-2 RNAi-treated embryos, the number of DYN-1-GFP-labeled foci at the cortex is reduced significantly (Figure 2, C and D; p values in legend). In addition, there is a reduction in the cytoplasmic background fluorescence observed. To determine whether the disruption in DYN-1-GFP foci to the cortex was specific to Arp2/3-regulated actin dynamics, we again disrupted WAVE function through gex-3 RNAi and quantified the number of DYN-1 foci at the cortex (Figure 2, C and D). We found that disruption of WAVE/SCAR function by gex-3 RNAi also significantly reduces the number of DYN-1-GFP cortical foci, similar to arx-2 RNAi, suggesting that localization of DYN-1 cortex relies on Arp2/3 and WAVE/SCAR-mediated actin dynamics."
• Remark: (Figure 2C,2D) arx-2 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000200	arx-2	K07C5.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K07C5.1	K07C5.1	1188	V: 10335776-10335949

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040933

0 Strain