WormMine: RNAi WBRNAi00107831

• WormBase ID: WBRNAi00107831
• Genotype: GFP::LGG-1
• Phenotype Remark: "Since many tumors require drastic changes in metabolism and particularly in catabolism to grow, we asked whether autophagy is induced in germline tumors in C. elegans. To test this, we examined whether autophagy-related proteins are expressed in the wild-type gonad and/or in the gld-1 germline tumor. We used transgenic animals that express fluorescence tagged versions of the following proteins: GFP::LGG-1 (GABARAP ortholog), GFP::LGG-2 (LC3 ortholog), and BEC-1::GFP (BECN1 ortholog). Consistent with previous reports, we observed that autophagy-related proteins are highly expressed in the nervous system, pharynx, intestine, hypodermis, vulva, spermatheca and somatic gonad of wild-type animals, but not in wild-type germline cells (Fig 1B). However, cells within the germline tumor in gld-1 RNAi animals show high expression of the autophagy pathway proteins from the first day of adulthood throughout their life span (Fig 1C and D). Notably, these transgenes (with the exception of BEC-1::GFP) are not expressed in wild-type germlines and only become activated during zygotic gene activation in embryos (Fig 1B) or somatic trans-differentiation in the tumor... Importantly, germline tumor cells show a heterogenous pattern of GFP::LGG-1 expression, with many cells showing elevated cytoplasmic expression and about 90% of them contain clearly discernible autophagosomes with rather uniform size of around 0.5 μm (Fig 2B)... Moreover, many (tumorous germ line) cells expressing GFP::LGG-1 have elongated protrusions which are often connected to neighboring cells, thereby resembling neurons in their shape (Fig 2C)."
• Remark: (Figure 1B-D,2B) gld-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001595	gld-1	T23G11.3	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:T23G11.3	T23G11.3	1392	I: 7695696-7695884

0 Laboratories

2 Phenotype

Identifier	Name	Description
WBPhenotype:0001276	ectopic expression transgene	Any variation in the cellular expression of a gene product produced by exogenous DNA that has been introduced into the organism such that it now expresses in cells/tissues not seen when compared to the expression of that gene product in control animals.
WBPhenotype:0000533	cell morphology variant	Any variation in the form or composition of the basic structural and functional unit of all organisms compared to control. Includes the plasma membrane and any external encapsulating structures such as the cell wall and cell envelope.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00049105

0 Strain