WormMine: RNAi WBRNAi00091272

• WormBase ID: WBRNAi00091272
• Genotype: unc-119(ed3) III; cbgIs98[pPept-1:GFP::rab-11.1;unc-119(+)]
• Phenotype Remark: Upon par-5 RNAi, GFP::RAB-11 labelled recycling endosomes (REs) cluster peripherally around or closely to ectopic F-actin structures (as determined by fluorescent (Rhodamine) phalloidin staining) at the basolateral cortex, in intestinal cells. The intestinal endotube labelled by apical F-actin can become more dilated and irregularly shaped on par-5 RNAi.
• Remark: (Figure S4i,j, 4b,d) M117.2/par-5 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003920	par-5	M117.2	Caenorhabditis elegans
WBGene00010919	M117.3	M117.3	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:M117.2b	M117.2b	381	IV: 11820991-11821284
CDS:M117.2a	M117.2a	747	IV: 11820991-11821284
CDS:M117.3	M117.3	378	IV: 11823516-11823602

1 Laboratories

Primary Identifier
MZE

5 Phenotype

Identifier	Name	Description
WBPhenotype:0000436	protein subcellular localization variant	Any variation in the placement of a protein within in a cell or cellular compartment compared to control.
WBPhenotype:0001587	actin organization biogenesis phenotype	Animals exhibit variations in the formation and/or arrangement of actin, an abundant cytoskeletal protein in most cells, often linked to the plasma membrane and concentrated at cell junctions compared to control (Wormatlas).
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0000710	intestinal morphology variant	Animals exhibit variations in the form, structure or composition of the chain of cuboidal cells which form a central lumen lined by microvilli that allow the passage of food, compared to control. Food passes from the posterior pharynx to the intestine where it is digested and then on to the rectum which processes the waste products for excretion (Wormatlas).
WBPhenotype:0002107	RAB-11 recycling endosome localization variant	Any variation in the subcellular localization of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00041129

0 Strain