WormMine: RNAi WBRNAi00091275

• WormBase ID: WBRNAi00091275
• Genotype: unc-119(ed3) III; cbgIs98[pPept-1:GFP::rab-11.1;unc-119(+)]
• Phenotype Remark: RNAi of unc-60 (ADF-cofilin) causes ectopic, peripheral microfilaments (F-actin, as determined by fluorescent (Rhodamine) phalloidin staining) and apically clustered GFP-RAB-11 labelled recycling endosomes (REs) in enterocytes.
• Remark: (Figure S4r,s) C38C3.5/unc-60 RNAi; Authors report that the unc-60a/b feeding clone was a gift from Carrie Cowan (Research Institute of Molecular Pathology, Vienna); cDNA coding sequence (C38C3.5b) used for curation

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006794	unc-60	C38C3.5	Caenorhabditis elegans
WBGene00302980	F53E2.2	F53E2.2	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F53E2.2	F53E2.2	498	V: 1475139-1475141
CDS:C38C3.5	C38C3.5	459	V: 1475139-1475141

1 Laboratories

Primary Identifier
MZE

4 Phenotype

Identifier	Name	Description
WBPhenotype:0000436	protein subcellular localization variant	Any variation in the placement of a protein within in a cell or cellular compartment compared to control.
WBPhenotype:0001587	actin organization biogenesis phenotype	Animals exhibit variations in the formation and/or arrangement of actin, an abundant cytoskeletal protein in most cells, often linked to the plasma membrane and concentrated at cell junctions compared to control (Wormatlas).
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0002107	RAB-11 recycling endosome localization variant	Any variation in the subcellular localization of membrane bound organelles, known as recycling endosomes, positive for the RAB protein RAB-11, that traffic material from early endosomes back to the plasma membrane of the cell, thereby recycling recently endocytosed material to be reused at the plasma membrane.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00041129

0 Strain