WormMine: RNAi WBRNAi00090741

• WormBase ID: WBRNAi00090741
• Phenotype Remark: The lifespan of worms from the F1, F2 and F3 generations in which ash-2 had been knocked down only in the P0 parental generation was significantly extended compared to that of descendants of worms treated with empty vector control in the P0 parental generation. By contrast, F4 generation descendants no longer had extended lifespan. Histone H3 Lysine 4 trimethylation (H3K4me3) levels were reduced in the P0 generation but not in subsequent generations, as determined by Western blot and antibody staining of ash-2 RNAi-treated animals.
• Remark: (Figure 3D-H, S7A,B,C, Table S2) ash-2 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00012458	ash-2	Y17G7B.2	Caenorhabditis elegans

4 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y17G7B.2a	Y17G7B.2a	1713	II: 11980475-11980545
CDS:Y17G7B.2e	Y17G7B.2e	1722	II: 11980475-11980545
CDS:Y17G7B.2b	Y17G7B.2b	1719	II: 11980475-11980545
CDS:Y17G7B.2d	Y17G7B.2d	1728	II: 11980475-11980545

1 Laboratories

Primary Identifier
ABR

3 Phenotype

Identifier	Name	Description
WBPhenotype:0000425	antibody staining reduced	Any variation that results in a decrease in the characteristic expression of a gene product, as detected by antibody staining, compared to control animals.
WBPhenotype:0000061	extended life span	The duration of viability in the adult phase of the life-cycle surpasses that of control animals.
WBPhenotype:0002078	histone methylation variant	The pattern or state of methyl modifications of histone varies from that of controls.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040327

1 Strain

WormBase ID
WBStrain00000001