WormMine: RNAi WBRNAi00106396

• WormBase ID: WBRNAi00106396
• Genotype: unc-45(e286)
• Phenotype Remark: "RNAi knockdown of chaperones was next performed with unc-45(ts) mutant strains under permissive conditions. Age synchronized L1 unc-45(e286) mutant animals kept at permissive conditions (15°C) were treated with RNAi for different molecular chaperones and monitored for motility defects. We first sought chaperones that cause unc-45(e286)-specific phenotypes at permissive conditions when depleted (Figure 2A). Of the 96 RNAi constructs tested, five genes induced loss of coordination and/or an egg-laying defect, resulting in reduced motility in more than 70% of the unc-45(e286) mutant animals. As noted above, down-regulation of unc-45 or unc-23 also induced a loss of coordination in wild-type animals (Table 1); these were discarded from the candidate gene list (Figure 2A). The remaining chaperones identified in the screen are CeHsp90 (daf-21), CeHop (sti-1) and CeAha1 (C01G10.8) (Figures 2B,C)." "CeHop and CeAha1 RNAi only affected unc45(e286)-expressing animals, where complete paralysis resulted (Figures 2B,C)."
• Remark: (Figure 2) sti-1/CeHop RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00019983	sti-1	R09E12.3	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:R09E12.3	R09E12.3	963	V: 773591-773602

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000644	paralyzed	Animals do not move and are not responsive to external mechanical stimulation.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00046852

0 Strain