WormMine: RNAi WBRNAi00111077

• WormBase ID: WBRNAi00111077
• Genotype: Pdat-1::GFP
• Phenotype Remark: Authors observed that sir-2.1 silenced worms raised on control diet and on reduced calorie diet showed alone no significant effect on GFP expression in DA neurons of the (Pdat-1::GFP) strain (Figs. 1E and F and 2A).The GFP expression pattern however exhibited a 2-fold reduction compared to sir-2.1 silenced worms when sir-2.1 knocked down worms were exposed to 6-OHDA (Figs. 1E-H and 2A). When the sir-2.1 knocked down worms, exposed to 6-OHDA were raised on a reduced calorie diet, the degeneration of DA neurons was not prevented as the GFP expression was statistically insignificant between the two groups. Hence, Sir-2.1 is necessary for achieving the protective effect of calorie restriction on dopaminergic neurodegeneration.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004800	sir-2.1	R11A8.4	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:R11A8.4b	R11A8.4b	1734	IV: 10363486-10363614
CDS:R11A8.4a	R11A8.4a	1824	IV: 10363486-10363614

0 Laboratories

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000612	nervous system physiology variant	Animals exhibit variations in any physical or chemical process required for cells as a collective unit of the nervous system to carry out their normal functions or activities or be able to perceive and respond to stimuli, compared to control animals.
WBPhenotype:0000636	neuron degeneration variant	Any variation in the ability of neurons to undergo a dramatic decrease in cell volume, collapse of the vacuoles and processes, and complete disintegration of the plasma membrane and most organelles under conditions that ellicit this response in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040154

0 Strain