WormMine: RNAi WBRNAi00088821

• WormBase ID: WBRNAi00088821
• Genotype: [GFP::PAR-2]
• Phenotype Remark: GFP::PAR-2 was mislocalized in embryos treated with arx-2 RNAi; "Cortical Z-series projections revealed that in wild-type or control embryos expressing GFP-PAR-2 (Schonegg et. al, 2007), PAR-2 is recruited to the posterior cortex as the posterior end of the embryo begins smoothing. On average, 43.8 +/- 4.28% (n = 5 embryos) of the cortical area is occupied by GFP-PAR-2 by pseudocleavage formation (Figure 1, C and D, PN meeting - 4:00). This area decreases slightly as the embryo progresses through the maintenance phase, occupying 39.78 +/- 7.57% (n = 5 embryos) of the total cortical area at pronuclear meeting and remaining steady through 4:00 min after pronuclear meeting (Figure 1C). In arx-2 RNAi-treated embryos, the area occupied by GFP-PAR-2 appears unaffected during establishment phase; however, PAR-2 expands beyond the typical domain during the maintenance phase to occupy almost 60% of the total cortical area over the course of multiple time points (Figure 1, C and D; wild type [WT]. n = 5 embryos; arx-2 RNAi, n = 5 embryos; p values in Figure 1D legend). However, before cell division, GFP-PAR-2 retracts back to a normal cortical domain and the embryo divides asymmetrically, likely due to a rescue mechanism that takes place during mitosis (Cai et. al, 2003; Siegrist and Doe, 2007; Schenk et. al, 2010)."
• Remark: (Figure 1C,1D) arx-2 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000200	arx-2	K07C5.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K07C5.1	K07C5.1	1188	V: 10335776-10335949

1 Laboratories

Primary Identifier
MAD

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040933

0 Strain