WormMine: RNAi WBRNAi00088829

• WormBase ID: WBRNAi00088829
• Phenotype Remark: In rack-1(RNAi) embryos, the establishment of PAR-6 foci was not affected, as PAR-6-GFP was normally enriched in the anterior half of the embryo by pseudocleavage formation (Fig. 1E). However, during pronuclear centration and rotation, PAR-6-GFP was not restricted to the anterior cortex but expanded into the posterior half of the cortex (4/7 embryos, Fig. 1F; Video S2). The boundary of PAR-6-GFP was sometimes rotated (2/7 embryos). The expansion or rotation of the PAR-6 domain was corrected during anaphase, just prior to furrow initiation, when PAR-6-GFP localization was indistinguishable from that observed in control embryos. The cleavage furrow was properly placed (Fig. 1G). We also noticed clumps of PAR-6-GFP foci during maintenance phase and furrow formation (Fig. 1F, G), which might suggest failures in PAR-6 trafficking or recycling.
• Remark: rack-1 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00010556	rack-1	K04D7.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K04D7.1	K04D7.1	978	IV: 10176710-10176824

1 Laboratories

Primary Identifier
MAD

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00038375

1 Strain

WormBase ID
WBStrain00043586