WormMine: RNAi WBRNAi00088832

• WormBase ID: WBRNAi00088832
• Genotype: [GFP::TBB-2; GFP::PAR-2; mCherry::PAR-6]
• Phenotype Remark: In rack-1(RNAi) embryos (Fig. 4D-F; Video S8), localization of PAR proteins during establishment phase was not unlike control embryos (Fig. 4D). During maintenance phase, however, the boundaries between the PAR-6- and PAR-2-occupied domains shifted and rotated to positions not perpendicular to the AP axis (Fig. 4E and 5C). During anaphase, the boundaries between PAR-6 and PAR-2 rotated back to wild-type positions, and the furrow formed at the correct position (Fig. 4F). Although we occasionally observe embryos showing expansion of the PAR-6 domain and shrinkage of the PAR-2 domain in these mid-focal-plane images and in cortical images from this same strain, we did not discover a significant change in average PAR domain asymmetry between rack-1(RNAi) and control embryos.
• Remark: rack-1 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00010556	rack-1	K04D7.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K04D7.1	K04D7.1	978	IV: 10176710-10176824

1 Laboratories

Primary Identifier
MAD

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0001588	microtubule organization biogenesis variant	Animals exhibit variations in the form, structure, composition or arrangement of a robust macromolecular array, composed primarily of tubulin, which forms long extended cytoskeletal elements compared to control (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00038375

0 Strain