WormMine: RNAi WBRNAi00088834

• WormBase ID: WBRNAi00088834
• Genotype: [GFP::TBB-2; GFP::PAR-2; mCherry::PAR-6]
• Phenotype Remark: In tbb-2(RNAi) embryos, the microtubules were still nucleated at the centrosomes but failed to elongate throughout the cell cycle (Fig. 4J-L; Video S10). In these embryos, polarity was still successfully established (Fig. 4J) but failed to be stabilized during maintenance phase, as shown by the shift of boundaries between the PAR-6 and PAR-2 domains (Fig. 5E).
• Remark: tbb-2(RNAi)

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006537	tbb-2	C36E8.5	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C36E8.5	C36E8.5	1353	III: 4016066-4016259

1 Laboratories

Primary Identifier
MAD

8 Phenotype

Identifier	Name	Description
WBPhenotype:0001152	pronuclear migration defective early emb	Pronucleus migration is abnormal in the inviable embryo.
WBPhenotype:0000034	embryonic polarity variant	Contents or structures of an embryonic cell are not restricted/localized to the same positions (or poles) compared with control animals.
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0001588	microtubule organization biogenesis variant	Animals exhibit variations in the form, structure, composition or arrangement of a robust macromolecular array, composed primarily of tubulin, which forms long extended cytoskeletal elements compared to control (Wormatlas).
WBPhenotype:0000766	centrosome pair and associated pronuclear rotation variant	Any variation in the rotation of the centrosome-pronuclear complex after its migration toward the center of the embryo, compared to control.
WBPhenotype:0000761	spindle position orientation defective early emb	Early embryos exhibit defects in the placement or the alignment of the array of microtubules and associated molecules that form between opposite poles of a eukaryotic cell during mitosis or meiosis.
WBPhenotype:0002501	ectopic cleavage furrow early emb	One or more cells of the early embryo (one-cell stage to four-cell stage) exhibit one or more cleavage furrows in locations not normally found in control cells.
WBPhenotype:0001158	pronuclear breakdown asynchronous early emb	Instead of breaking down synchronously, the two pronuclei break down asynchronously in the inviable one-cell embryo.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00038375

0 Strain