WormMine: RNAi WBRNAi00108414

• WormBase ID: WBRNAi00108414
• Genotype: IFB-1A::GFP (juIs176)
• Phenotype Remark: Analysis of smo-1(RNAi) viable embryos revealed an abnormal pattern of IFB-1 expression (Figures 4I-4P). As soon as GFP fluorescence could be detected at the comma stage and later, at 1.5-fold stage, IFB-1 was localized to abnormal thick and short filamentous structures in all regions of the epidermis (detected in 34/38 embryos analyzed) while cytoplasmic staining was very weak or undetectable (32/38 embryos). Authors suggest that SUMO is required to keep IFB-1 in a soluble fraction that serves as storage for free subunits necessary for filament formation during elongation.
• Remark: Authors used the feeding-RNAi approach to deplete SUMO and selected for embryos that had enough SUMO to perform embryonic cell divisions until the comma stage (early elongation).

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004888	smo-1	K12C11.2	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K12C11.2	K12C11.2	276	I: 1340703-1340768

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00035491

0 Strain