WormMine: RNAi WBRNAi00086890

• WormBase ID: WBRNAi00086890
• Phenotype Remark: 91 to 100% of the embryos failed to hatch, RNAi of Ran significantly reduced the brood size. When Ran is depleted by RNAi, several features are striking (4 of 5 embryos). First, the pronuclei are very small. Second, the embryos fail to set up a spindle, although the centrosomes are still capable of nucleating astral microtubules. Third, from being closely associated with the male pronucleus the centrosomes immediately move far apart and after several minutes undergo strong sideward movements, as in normal anaphase. This process (spindle rocking) together with cleavage furrow ingression was used throughout this study to define anaphase in embryos where no spindle was formed. Fourth, in some embryos, the centrosomes are not properly positioned so that the first cleavage leads to an abnormal bisection of the embryo where the posterior cell P1 is larger than the anterior AB. Taken together, these data indicate that Ran is required for spindle formation, but not for astral microtubule nucleation.
• Remark: Full length C. elegans Ran (ran-1, nucleotides [nt] 1 to 648 of predicted open reading frame [ORF]) was used for RNAi. only intron-less sequences were amplified.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004302	ran-1	K01G5.4	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K01G5.4	K01G5.4	648	III: 10746890-10746982

0 Laboratories

5 Phenotype

Identifier	Name	Description
WBPhenotype:0000154	reduced brood size	The characteristic number of offspring produced is lower compared to that of control animals.
WBPhenotype:0000062	lethal	Animals die prematurely at any stage of the life cycle.
WBPhenotype:0001151	pronucleus centrosomes defective early emb	Pronucleus is abnormal in the inviable embryo.
WBPhenotype:0001792	nuclei small	Nuclei of specific cells are smaller compared to control animals.
WBPhenotype:0000759	spindle defective early emb	Early embryos exhibit defects in the assembly, disassembly, arrangement, elongation or stabilization of the microtubules and associated molecules that form between opposite poles of a eukaryotic cell during a cell cycle.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005637

1 Strain

WormBase ID
WBStrain00000001