WormMine: RNAi WBRNAi00106289

• WormBase ID: WBRNAi00106289
• Phenotype Remark: "To specifically study DCC mediated X repression, we analyzed gene expression changes in hermaphrodites mutant for dpy-27 or upon dpy-27 RNAi knockdown. As mutants and RNAi treated worms showed more variability in staging, we mainly used 'mixed stage embryos' isolated by bleaching gravid adults. Mixed stage embryos contained 100-300 cells (S4A Fig). We used dpy-27 RNAi in mixed embryos and L3, because of the difficulty collecting dpy-27(y56) null mutant. Western blot analyses showed ~70% and ~40% knockdown of DPY-27 in embryos and L3s, respectively (S4B Fig). Mutation (Fig 3D) or depletion (Fig 3E) of dpy-27 caused significant X chromosome derepression in early and mixed-stage embryos, L1 and L3 worms."
• Remark: dpy-27 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001086	dpy-27	R13G10.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:R13G10.1	R13G10.1	4410	III: 3813909-3814171

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000718	dosage compensation variant	Variations in the process of normalizing the expression level of X-linked genes between XX and XO animals compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00048953

0 Strain