WormMine: RNAi WBRNAi00087594

• WormBase ID: WBRNAi00087594
• Phenotype Remark: Even after injecting high concentrations of lem-2 dsRNA, residual (slightly punctate) nuclear envelope staining of Ce-MAN1 was still detectable in lem-2(RNAi) embryos. Reduced Ce-MAN1 had no effect on the localization of Ce-lamin, Ce-emerin, or an unrelated nuclear membrane protein, UNC-84. Quantitative analysis revealed a 6- to 10-fold decrease in the fluorescence intensity of Ce-MAN1 in lem-2(RNAi) embryos, relative to control embryos. Nonetheless, this 8590% reduction of Ce-MAN1 pro-tein caused 15% embryonic lethality (n = 200), with most dead embryos arresting after the 2-fold stage. DAPI staining revealed that in a few (<1%) early lem-2(RNAi) embryos, pairs of daughter cells were connected by a thin bridge of chromatin. Indirect immunofluorescence staining suggested that Ce-lamin localized correctly around the chromatin masses, except at the sites of chromatin bridges. However, the majority of lem-2(RNAi) embryos had normal mitosis and developed into normal fertile adults.
• Remark: dsRNA corresponding to Ce-MAN1 residues 147 to 385 was used. The low-penetrance chromosome segregation defect was unlikely to be due to mislocalization of other nuclear envelope proteins, because no effects was observed on Ce-lamin, Ce-emerin, or UNC84. Instead, the embryonic lethality and chromosome segregation defects seen in lem-2(RNAi) embryos, which retained 1015% Ce-MAN1 protein, suggested that Ce-MAN1 might be an essential component of the nuclear envelope, with roles in cell division or early development.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00002275	lem-2	W01G7.5	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:W01G7.5	W01G7.5	1503	II: 14063455-14063595

0 Laboratories

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0000773	chromosome segregation variant	Any variation in the processes that regulate the apportionment of chromosomes to each of two daughter cells compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005828

1 Strain

WormBase ID
WBStrain00000001