WormMine: RNAi WBRNAi00110473

• WormBase ID: WBRNAi00110473
• Genotype: mex-3, pop-1 double RNAi
• Phenotype Remark: "Hermaphrodites heterozygous for the balanced hlh-1(cc450) null allele (Chen et al., 1992) were treated with mex-3, pop-1 double RNAi and progeny embryos collected. If HLH-1 was required for PAL-1 to induce muscle, 25% of the embryos (corresponding to the homozygous hlh-1(cc450) animals) should not respond to PAL-1. However, all treated embryos resulted in widespread body wall muscle-like myogenesis demonstrating that HLH-1 activity was not required for PAL-1-induced myogenesis (Fig. 7). We did notice that 22% (n=196) of the embryos showed less robust myogenesis, based on MHC A filament formation (Fig. 7B). These experiments were repeated using the temperature-sensitive hlh-1 allele cc561 (Harfe et al., 1998a), or hlh-1 RNAi for which the genotype of each embryo was unambiguous and the same results were obtained (Fig. 7C); myogenesis occurred but was not as robust in the absence of HLH-1."
• Remark: hlh-1 RNAi. Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001948	hlh-1	B0304.1	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:B0304.1a	B0304.1a	963	II: 4519069-4519238
CDS:B0304.1b	B0304.1b	975	II: 4519069-4519238
CDS:B0304.1c	B0304.1c	960	II: 4519069-4519238

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000861	body wall muscle development variant	Any variation in the progression of the principal muscle cell type whose contractile activity generates body motion in the nematode over time compared to control. In C. elegans they consist of 95 unfused cells in the adult organized into four muscle quadrants. Their sarcomeres are obliquely striated and lie lengthwise along the body wall (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00025033

0 Strain