WormMine: RNAi WBRNAi00092476

• WormBase ID: WBRNAi00092476
• Phenotype Remark: Injection of pop-1 dsRNA results in the production of arrested embryos with approximately double the wild-type number of gut cells. Almost all of these embryos (299/318 = 94%) fail to express transgenic LacZ (under the control of a modified ges-1 promoter) in the gut (unlike wild type embryos). Although the endogenous ges-1 gene is still expressed, the anterior-to-posterior gradient of endogenous ges-1 activity observed in wild-type embryos is now abolished.
• Remark: (Text, Figure 9) pop-1 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00004077	pop-1	W10C8.2	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:W10C8.2	W10C8.2	1317	I: 2823468-2823937

1 Laboratories

Primary Identifier
JM

4 Phenotype

Identifier	Name	Description
WBPhenotype:0000867	embryonic arrest	Cessation of development during any stage occurring after fertilization and until hatching compared to control.
WBPhenotype:0001375	transgene expression undetectable	The expression of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism is reduced below the threshold of detection.
WBPhenotype:0000124	enzyme activity reduced	Any variation that results in a decrease in the catalytic activity of an enzyme compared to control. Catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein (can include an RNA component).
WBPhenotype:0001636	excess intestinal cells	Animals contain an excess number of intestinal cells compared to control. In C. elegans, intestinal cells are derived from E-blastomere. The overproduction of intestinal tissues is a result of other blastomeres adopting an E-like fate or excess cell proliferation in the E lineage.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00003331

0 Strain