WormMine: RNAi WBRNAi00093341

• WormBase ID: WBRNAi00093341
• Phenotype Remark: Reducing TOMM-40 levels, by exposing 4th stage larvae to feeding RNAi, causes a growth retardation in their progeny with most animals arresting as embryos or between the 1st and the 3rd larval stages (Figure 3A). As an index of the growth defect, the number of larvae in the 1st larval stage (L1) was counted 24 hours after removing their mothers in a feeding RNAi assay. While only 0.4% (n = 223) of animals fed with empty vector (RNAi) bacteria were in stage L1, 60% (n =194) of tomm-40(RNAi) animals were in this stage. Later, most of the larvae appeared to arrest permanently at the L2 or L3 stage although many still remained in the L1 stage. Using high power microscopy, we could not detect any catastrophic developmental defects that might cause the larval arrest.
• Remark: (Figure 3A) tomm-40 RNAi

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007686	tomm-40	C18E9.6	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C18E9.6	C18E9.6	906	II: 8972375-8972498

1 Laboratories

Primary Identifier
GOT

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0000059	larval arrest	The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00038075

0 Strain