WormMine: RNAi WBRNAi00065157

• WormBase ID: WBRNAi00065157
• Genotype: pha-4(zu225)
• Phenotype Remark: Enhancement of pha-4(zu225) L1 lethality and RNAi in this background causes a pharynx unattached (pun) phenotype.
• Remark: pha-4(zu225) is a temperature sensitive allele and 24 C is a permissive temperature. The enhancement of pha-4 lethality was shown to have some specificity as no enhancement was seen in either the hsf-1(sy441), tbx-2(ut180) or lin-26(n156) backgrounds. In addiition this RNAi did not enhance the movement defect in the unc-54(r293ts) allele. Like pha-4(zu225), RNA derived from unc-54(r293) is subject to degradation by the nonsense-mediated decay pathway.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

4 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00007027	ssl-1	Y111B2A.22	Caenorhabditis elegans
WBGene00013740	hut-1	Y111B2A.20	Caenorhabditis elegans
WBGene00220229	Y111B2A.35	Y111B2A.35	Caenorhabditis elegans
WBGene00220230	Y111B2A.36	Y111B2A.36	Caenorhabditis elegans

5 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y111B2A.20b	Y111B2A.20b	141	III: 12713527-12713667
CDS:Y111B2A.22b	Y111B2A.22b	5649	III: 12714588-12714868
CDS:Y111B2A.20a	Y111B2A.20a	1023	III: 12711560-12711693
CDS:Y111B2A.22a	Y111B2A.22a	7188	III: 12714588-12714868
CDS:Y111B2A.22d	Y111B2A.22d	6750	III: 12715723-12715899

1 Laboratories

Primary Identifier
SM

2 Phenotype

Identifier	Name	Description
WBPhenotype:0001748	pharynx unattached	The pharynx or pharyngeal muscle does not form a functional connection to the mouth or buccal cavity.
WBPhenotype:0000062	lethal	Animals die prematurely at any stage of the life cycle.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00028549

0 Strain