RNAi phenotype in lin-35(n2239) worms was reproducibly stronger than those observed in WT (primary screen) and rrf-3(pk1426) (secondary screen) animals.
Remark
candidate lin-35 interacting gene based on results of the secondary screen
Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.