(Figure S2ac) acs-1(RNAi)56 (targeting acs-1 3'UTR; effectively intestine-specific RNAi, as this RNAi treatment did not affect acs-1 expression in the somatic gonad)
Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.