WormMine: RNAi WBRNAi00061169

• WormBase ID: WBRNAi00061169
• Phenotype Remark: The first furrow is reduced, but there is no signal from the midzone.
• Remark: Asymmetric spindle severing assay: at the onset of anaphase one aster is separated from its associated chromatin using an ultraviolet laser, creating a cell with one isolated aster and the other aster still attached to the midzone. Asymmetric spindle severing spatially separates the spindle midzone and the region midway between the asters, leading to the generation of two furrows. Furrow ingression started midway between the asters (first furrow). However, the furrow did not complete midway between the asters: a second furrow formed at the cell cortex closest to the midzone, the two distinct furrows then met and cytokinesis completed.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00006974	zen-4	M03D4.1	Caenorhabditis elegans

6 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:M03D4.1a	M03D4.1a	2328	IV: 6118224-6118666
CDS:M03D4.1b	M03D4.1b	2319	IV: 6118224-6118666
CDS:M03D4.1d	M03D4.1d	2364	IV: 6118224-6118666
CDS:M03D4.1f	M03D4.1f	2358	IV: 6118224-6118666
CDS:M03D4.1c	M03D4.1c	2334	IV: 6118224-6118666
CDS:M03D4.1e	M03D4.1e	2325	IV: 6118224-6118666

1 Laboratories

Primary Identifier
TH

2 Phenotype

Identifier	Name	Description
WBPhenotype:0001129	cleavage furrow defective early emb	Embryos exhibit defects in the formation, maintenance or termination of the groove in the cell surface near the old metaphase plate during cytokinesis.
WBPhenotype:0001018	cytokinesis variant emb	Any variation that disrupts the cytoplasmic division of a cell during embryonic development compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00026732

1 Strain

WormBase ID
WBStrain00000001