WormMine: RNAi WBRNAi00114743

• WormBase ID: WBRNAi00114743
• Genotype: drIs34[myo-3p::(PR)50-GFP; myo-3p::mCherry]
• Phenotype Remark: "we identified two RNAi knockdowns that were able to suppress the loss of motility and arrest in growth caused by (PR)50. These two hits were subsequently screened six additional times, all of which suppressed (PR)50 growth arrest and paralysis. Both RNAi clones were sequenced and the sequences were aligned to the C. elegans genome using BLAST to identify the affected genes. The two genes uncovered in the screen were lin-54 and dss-1. In most assays, dss-1(RNAi) had a much stronger toxicity suppression phenotype than lin-54(RNAi) and rescued motility back to near wildtype levels. Because the dss-1(RNAi) phenotype was robust, additional mechanistic studies were performed to understand how dss-1 contributes to DPR toxicity... Two independent paralysis assays showed that animals on dss-1(RNAi) exhibited reduced paralysis compared to the negative control EV(RNAi) (Figure 1B)... This decrease in C. elegans age-dependent paralysis following dss-1(RNAi) shows that dss-1 normally functions to facilitate (PR)50 and (GR)50 induced age-dependent paralysis... Two independent VSA [video speed analysis] trials showed increased motility in (PR)50 expressing animals on dss-1(RNAi) compared to EV(RNAi) (Figure 1D, F)... Results from this experiment showed that C. elegans increased in size on dss-1(RNAi) compared to EV(RNAi), showing that dss-1(RNAi) suppressed the growth arrest caused by (PR)50 (Figure 1H). This result shows that dss-1 normally functions to help facilitate (PR)50-induced suppression of growth."
• Remark: dss-1 (WBGene00022492) RNAi, ORFeome clone

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022492	dss-1	Y119D3B.15	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:Y119D3B.15	Y119D3B.15	249	III: 1194668-1194805

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0002605	dipeptide repeat protein toxicity suppressed	Normally toxic dipeptide repeat proteins appear to be less toxic to animals, compared to control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00059748

0 Strain