WormMine: RNAi WBRNAi00107835

• WormBase ID: WBRNAi00107835
• Phenotype Remark: "gld-1 germline tumors do not only comprise germ cells but also ectopically differentiated somatic cells, such as muscle, neurons and intestinal cells (Fig 1A and S1A). We observed that the morphology of cells expressing autophagy markers within the germline tumor resembles the morphology of neuronal cells (Figs 1 and 2C). Therefore, we used a C. elegans strain expressing a pan-neuronal marker (UNC-119::mCherry) and GFP::LGG-1. Confocal microscopy of germline tumors induced by gld-1 RNAi, showed that LGG-1 is expressed in cells differentiating as neurons (Fig 4A). Concomitantly, we examined differentiation of other somatic cell types in gld-1 germline tumors, by knocking down gld-1 in reporter strains expressing GFP or mCherry under the control of tissue-specific -- neuronal (unc-119), muscle (myo-3) or pharyngeal (pha-4) -- promoters. Although gld-1-deficient germ cells reprogram mainly as neurons, we also observe -- to a lesser extent -- differentiation of muscle and pharynx cells (Fig S1A)."
• Remark: (Figures 1,2,S1) gld-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001595	gld-1	T23G11.3	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:T23G11.3	T23G11.3	1392	I: 7695696-7695884

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000414	cell fate transformation	The cell takes on a different identity from which it was initially committed.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00049105

0 Strain