WormMine: RNAi WBRNAi00087535

• WormBase ID: WBRNAi00087535
• Phenotype Remark: When treated with dsRNA from the capping enzyme gene (cel-1), 100% of the embryos failed to hatch. One- or two-cell cel1(RNAi) embryos contained cytoplasmic granules not usually seen in wild-type embryos. These granules, however, are commonly observed when other genes predicted to function in mRNA processing are tested. Despite the presence of aberrant cytoplasmic granules, other basic cell biological events appeared normal during the first embryonic cell divisions, consistent with the idea that these early stages are not dependent on newly transcribed genes. However, analysis of terminal phenotypes showed defects that included embryos with no gut granules (94%, n = 79) but variable levels of differentiation including twitching (a sign of muscle differentiation) and other cell types. Similar results were obtained by feeding dsRNA.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000466	cel-1	C03D6.3	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C03D6.3a	C03D6.3a	1872	I: 9665311-9665391
CDS:C03D6.3b	C03D6.3b	1722	I: 9665347-9665391

0 Laboratories

3 Phenotype

Identifier	Name	Description
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0001082	large cytoplasmic granules early emb	Abnormally large granules are observed in the cytoplasm of P0.
WBPhenotype:0000483	no gut granules	Animals lack the different inclusions within the intestinal cytoplasm, including birefringent crystalline objects and other dark-staining endosomes. In C. elegans these contents typically include glycogen granules (polysaccharide storage), rhabditin (carbohydrate storage), yolk granules (lipid and protein), and concentrated waste products (Wormatlas).

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005844

1 Strain

WormBase ID
WBStrain00000001