WormMine: RNAi WBRNAi00110387

• WormBase ID: WBRNAi00110387
• Phenotype Remark: "An anti-chondroitin sulfate antibody has been previously used to detect the presence of chondroitin (one of the secreted GAGs) on the cell surface of embryonic cells and gonad (Mizuguchi et al. 2003). Using this antibody, we observed the reported staining for wild-type embryos (Fig. 3P,Q) but did not detect the antigen on the acs-1(RNAi)C17ISO embryo cell surface (Fig. 3R,S). Biosynthesis of chondroitin was not disrupted in the acs-1(RNAi)C17ISO animals, as it was readily detectable in the gonads (Fig. 3T,U). These data suggested that the absence of the perivitelline space in acs-1(RNAi)C17ISO embryos may have been caused, at least in part, by defective exocytosis of GAGs."
• Remark: (Figure 3R, 3S) acs-1(RNAi)

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00018488	acs-1	F46E10.1	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F46E10.1c	F46E10.1c	1737	V: 6529269-6529439
CDS:F46E10.1a	F46E10.1a	1872	V: 6525095-6525136
CDS:F46E10.1b	F46E10.1b	1794	V: 6527511-6527520

0 Laboratories

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000425	antibody staining reduced	Any variation that results in a decrease in the characteristic expression of a gene product, as detected by antibody staining, compared to control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00040893

0 Strain