WormMine: RNAi WBRNAi00088417

• WormBase ID: WBRNAi00088417
• Phenotype Remark: Whereas ~8% of the F1 progeny arrested as unhatched embryos, the majority of progeny arrested as larvae in this case (37% L1-L2 arrest, and 22% L3-L4 arrest, n=328). The variable phenotypes observed were probably due to gene-dependent inefficacy inherent of RNAi experiments. These larvae showed no apparent morphological abnormality, but were affected severely in locomotion. Even F1 progeny that arrested as unhatched embryos seemed to have completed normal morphogenesis. Because expression of COH-1 became detectable at the mid-embryogenesis stage, and the RNAi-arrested embryos showed a significantly reduced level of COH-1, it seemed that the most rigorous loss-of-function phenotype for coh-1 would be late embryonic lethality after completion of morphogenesis. Authors next disrupted the function of COH-1 postembryonically by soaking L1 worms to a dsRNA solution and found that some coh-1(RNAi) adults were defective in egg laying. The observed defects in locomotion and egg laying implicated that the function of COH-1 might be important for proper function of muscle and/or the nervous system.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. Authors used yk226d1 (coh-1).

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000591	coh-1	K08A8.3	Caenorhabditis elegans

4 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K08A8.3d	K08A8.3d	156	X: 7466321-7466476
CDS:K08A8.3b	K08A8.3b	495	X: 7466321-7466491
CDS:K08A8.3a	K08A8.3a	1794	X: 7466321-7466491
CDS:K08A8.3c	K08A8.3c	414	X: 7466321-7466491

1 Laboratories

Primary Identifier
YM

4 Phenotype

Identifier	Name	Description
WBPhenotype:0000867	embryonic arrest	Cessation of development during any stage occurring after fertilization and until hatching compared to control.
WBPhenotype:0000643	locomotion variant	Animals exhibit deviations in self-propelled movement on a solid medium compared to control animals.
WBPhenotype:0000006	egg laying defective	Eggs are not laid, laid at a slower rate or eggs are laid at a later stage of development compared to control.
WBPhenotype:0000059	larval arrest	The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005937

1 Strain

WormBase ID
WBStrain00000001