WormMine: RNAi WBRNAi00106301

• WormBase ID: WBRNAi00106301
• Phenotype Remark: "... we first compiled a list of C-elegans chaperones (97 genes) (Table 1) and examined the effects of down-regulation of each chaperone in wild-type animals (Figure 2A). Down-regulation of genes encoding 10 chaperones (17 genes), including CTT, HSP-1 (Hsc70/HSPA8), HSP-4 (BIP), HSP-6 (Mortalin), and DAF-21 (Hsp90), resulted in severe organismal phenotypes, such as lethality, developmental arrest, or sterility (Table 1, Figure S2). In contrast, downregulation of genes encoding most chaperones examined had no apparent phenotypes (Table 1). Our data are in agreement with published RNAi screens for wild-type RNAi treatment (Table 1, data curated by wormbase). When knockout mutants or data from enhanced RNAi efficacy experiments, (for example via dsDNA [sic] injection into the gonads) were considered, more acute phenotypes were observed for some chaperones (Table 1, data curated by wormbase)." "As expected, down-regulation of only two chaperones, unc45 or unc-23, showed a marked movement defect. For example, based on their impact on muscular ultrastructure or their involvement in myopathies, molecular chaperones, such as TRIC (cct), alpha-B-crystallin/HSPB5 (hsp-25), and DNAJB6 (dnj-24), were suggested to play a key role in sarcomere assembly and maintenance (Ding and Candido, 2000; Vang et al., 2005; Kim et al., 2008; Willis et al., 2009; Willis and Patterson, 2010; Garrido et al., 2012; Haslbeck et al., 2012; Sarparanta et al., 2012). Although these chaperones are associated with the sarcomere (Leroux and Candido, 1997; Ding and Candido, 2000; Meissner et al., 2011), knockdown of their genes by feeding RNAi did not result in notable motility defects (Table 1)."
• Remark: (Table 1) cct-4 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000379	cct-4	K01C8.10	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:K01C8.10	K01C8.10	1623	II: 8283668-8283771

0 Laboratories

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000688	sterile	Animals generate defective gametes, are otherwise unable to reproduce or they generate progeny that are unable to reproduce.
WBPhenotype:0000059	larval arrest	The developmental program of the animals halts at any larval stage and thus fails to reach adulthood. In C.elegans, this occurs anytime during L1-L4 stage.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00046852

1 Strain

WormBase ID
WBStrain00000001