WormMine: RNAi WBRNAi00064966

• WormBase ID: WBRNAi00064966
• Genotype: unc-54::POLYQ(Q35)-YFP
• Phenotype Remark: RNAi caused early onset of foci formation (polyglutamine-YFP aggregation)
• Remark: In strains expressing unc-54::POLYQ(Q35)-YFP, a diffuse YFP staining pattern is seen in all expressing cells from the moment they hatch through young adulthood. Onset of aggregation is invariable and occurs after the animals reach adulthood at the fifth day after hatching. L1 larva were feed RNAi bacteria in liquid culture and animals were assayed for fluorescent foci three days post-L1.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

3 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00000063	act-1	T04C12.6	Caenorhabditis elegans
WBGene00000064	act-2	T04C12.5	Caenorhabditis elegans
WBGene00000065	act-3	T04C12.4	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:T04C12.6	T04C12.6	1131	V: 11081073-11081262
CDS:T04C12.4	T04C12.4	1131	V: 11072012-11072171
CDS:T04C12.5	T04C12.5	1131	V: 11076900-11077059

1 Laboratories

Primary Identifier
NL

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000679	transgene subcellular localization variant	Any variation in the subcellular localization of a gene product produced by DNA coded for by exogenous genetic material that has been introduced into the organism compared to its expression in control animals.
WBPhenotype:0001405	protein aggregation variant	Variations that result in the accumulation of soluble/insoluble protein aggregates in a particular cell or tissue, compared to control. Protein aggregates are often formed by interactions between misfolded protein molecules and/or deficiencies in proper protein folding.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00006510

0 Strain