WormMine: RNAi WBRNAi00077101

• WormBase ID: WBRNAi00077101
• Genotype: rrf-1(pk1417)
• Phenotype Remark: after mdf-1 RNAi treatment there was an increase in germ cell proliferation after starvation.
• Remark: The rrf-1 gene encodes an RNA-directed RNA polymerase (RdRP) homologue. Somatic cells (but not germ cells) carrying the homozygous rrf-1(pk1417) allele are resistant to RNAi treatment. Because there was an increase in germ cell proliferation after starvation in this experiment authors posit that the mdf-1 gene cell autonomously functions in starvation-induced cell cycle arrest of germ cell precursors. Exact sequence used for RNAi not stated by authors, genomic sequence of gene used for curation.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00003160	mdf-1	C50F4.11	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:C50F4.11	C50F4.11	2040	V: 9541945-9542074

1 Laboratories

Primary Identifier
RQ

3 Phenotype

Identifier	Name	Description
WBPhenotype:0000823	germline proliferation variant	Nuclear divisions in the germ line are either expanded are reduced compared to control.
WBPhenotype:0000147	organism starvation response variant	Any variation in the state or activity of an organism as a result of deprivation of nourishment, compared to control.
WBPhenotype:0000740	cell cycle variant	Cells of the animals exhibit variations in the initiation, progression or exit from the biochemical and morphological processes that occur during successive replication or nuclear replication events from that observed for control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00031557

0 Strain