WormMine: RNAi WBRNAi00078173

• WormBase ID: WBRNAi00078173
• Phenotype Remark: Worms displayed deficiencies in maintaining epithelial integrity and vacuolation of the hypodermis was observed. The degeneration of the hypodermis was confirmed using the membrane-impermeable dye Hoechst 33258, which fluoresces when bound to DNA and thus stains nuclei of permeable, but not impermeable, living cells. When cuti-1(RNAi) worms were suspended in buffer containing Hoechst 33258, all worms showed fluorescence of nuclei compared with none of controls. This confirms that silencing of cuti-1 expression causes the hypodermal membrane and cuticle to become highly permeable and no longer able to provide a protective barrier from the environment.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00022591	cuti-1	ZC328.1	Caenorhabditis elegans

1 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:ZC328.1	ZC328.1	609	I: 6404593-6404761

1 Laboratories

Primary Identifier
WT

2 Phenotype

Identifier	Name	Description
WBPhenotype:0000703	epithelial morphology variant	Variations in the form, composition or structure of the layer of cells that cover the external surface of an organism and form the inner lining between the tissues and organs of that organism and the external environment compared to control. In C. elegans epithelial cells include epithelium of the alimentary tract, intestinal cells, hypodermis, interfacial cells and somatic gonad cells (Wormatlas).
WBPhenotype:0001211	cuticle integrity variant	The physical strength or function of the cuticle varies from that observed for control animals.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00033062

0 Strain