WormMine: RNAi WBRNAi00088922

• WormBase ID: WBRNAi00088922
• Phenotype Remark: Nuclei from embryos treated with double RNAi against him-1 and either scc-1 or tim-1 had numerous FISH signals, indicating severe chromosome segregation defects and extensive aneuploidy.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, genomic sequence of gene used for curation. Primers used were him-1 5' GCGTAATACGACTCACTATAGGGAAGGCAGAGAACAACTCGACTCAG 3' and 5' CGCAATTAACCCTCACTAAAGGGATCAGCAGAACCTCCGGACATA 3', scc-1 5' TAATACGACTCACTATAGGGATCCATGGAACGGAAATTTCCCGTATAG 3' and 5' TAATACGACTCACTATAGGGCGGCCGCTCCAGCGAATCCACTCTGGATAGGG.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

2 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001860	him-1	F28B3.7	Caenorhabditis elegans
WBGene00004737	scc-1	F10G7.4	Caenorhabditis elegans

3 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:F28B3.7b	F28B3.7b	423	I: 4951810-4952031
CDS:F10G7.4	F10G7.4	1938	II: 4698721-4698972
CDS:F28B3.7a	F28B3.7a	3789	I: 4951810-4952031

1 Laboratories

Primary Identifier
TY

1 Phenotype

Identifier	Name	Description
WBPhenotype:0000773	chromosome segregation variant	Any variation in the processes that regulate the apportionment of chromosomes to each of two daughter cells compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005919

0 Strain