WormMine: RNAi WBRNAi00086810

• WormBase ID: WBRNAi00086810
• Genotype: lin-35(n745)I
• Phenotype Remark: This led to embryonic lethality in 88% of F1 progeny, with the remaining 12% arresting during early larval stages (n = 269). Nonviable embryos showed a variety of phenotypes ranging from premorphogenetic to late-stage arrest (Fig. 5C,D). In the majority of cases, arrested embryos showed clear signs of tissue-specific differentiation but failed to complete morphogenesis and contained vacuoles and necrotic regions (Fig. 5C,D). In addition, a significant percentage of embryos (30%) were misshapen or of abnormal size (Fig. 5D). We conclude that, in addition to controlling cell proliferation during postembryonic development, lin-35 and fzr-1 play essential, albeit redundant, roles during embryonic development.
• Remark: paper remark: Exact sequence used for RNAi not stated by authors, authors said fzr-1 750-bp genomic spanning exons 12 was used. Curator used 852 bp of fzr-1 genomic sequence (spanning exon 1-2) in WS227.

1 Organism

• Name: Caenorhabditis elegans
• Taxon Id: 6239

1 Data Sets

• WormBaseAcedbConverter

1 Inhibits Gene

WormBase Gene ID	Gene Name	Sequence Name	Organism
WBGene00001510	fzr-1	ZK1307.6	Caenorhabditis elegans

2 Inhibits Predicted Gene

WormMine ID	Sequence Name	Length (nt)	Chromosome Location
CDS:ZK1307.6a	ZK1307.6a	2109	II: 9640995-9641204
CDS:ZK1307.6b	ZK1307.6b	2115	II: 9640995-9641210

1 Laboratories

Primary Identifier
MH

5 Phenotype

Identifier	Name	Description
WBPhenotype:0000081	L1 arrest	The developmental program of the animals does not continue past the first larval stage (L1).
WBPhenotype:0000050	embryonic lethal	Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.
WBPhenotype:0000779	late embryonic arrest	Cessation of development during the final stages of embryogenesis. In C. elegans, this stage normally occurs 620-800 min after the first cleavage at 20C and is the stage after elongation.
WBPhenotype:0000361	lima bean arrest	Cessation of development at an intermediate stage in embryogenesis, immediately following the completion of gastrulation. In C. elegans this occurs around 350-390 minutes after first cleavage, cell number is at approximately 560 cells. At this time cell proliferation ceases and the embryo begins morphogenesis and elongation. The shape of the entire embryo within the eggshell resembles a lima bean (Wormatlas).
WBPhenotype:0001136	embryonic morphology variant	Animals exhibit variations in the form, structure or composition of any of its parts during any developmental stage ranging from zygote formation to hatching compared to control.

0 Phenotype _ Not _ Observed

1 Reference

First Author	Title	Year	Journal	Volume	Pages	PubMed ID
						WBPaper00005137

0 Strain