WormMine

WS294

Intermine data mining platform for C. elegans and related nematodes

RNAi :

WormBase ID  WBRNAi00116291 Phenotype Remark  Figure S1. 60-80% of embryos (F1) are lethal when parent population (P0) is fed daf-15 RNAi from hatching. Embryos that do hatch will arrest at L3 stage
Remark  daf-15 (WBGene00000911) From PMID:26109047/WBPaper00047025: "For the daf-15(RNAi) construct, a PCR fragment of daf-15 genomic DNA (71871 nucleotides [nt]) was inserted into the L4440 vector by EcoRI/KpnI". 800 nt from 5' end of daf-15 gene used for curation
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1 Organism

Name Taxon Id
Caenorhabditis elegans 6239

1 Data Sets

Name URL
WormBaseAcedbConverter  

1 Inhibits Gene

WormBase Gene ID Gene Name Sequence Name Organism
WBGene00000911 daf-15 C10C5.6 Caenorhabditis elegans

5 Inhibits Predicted Gene

WormMine ID Sequence Name Length (nt) Chromosome Location
CDS:C10C5.6c C10C5.6c 5403   IV: 9386208-9386272
CDS:C10C5.6a C10C5.6a 5331   IV: 9386464-9386538
CDS:C10C5.6d C10C5.6d 5340   IV: 9386464-9386538
CDS:C10C5.6b C10C5.6b 5349   IV: 9386464-9386538
CDS:C10C5.6e C10C5.6e 5358   IV: 9386464-9386538

0 Laboratories

1 Phenotype

Identifier Name Description
WBPhenotype:0000050 embryonic lethal Animals die during embryonic development. In C. elegans, often assayed as refractile eggs that fail to hatch; when applied to large-scale RNAi screens in C. elegans, more than 10% of embryos die.

0 Phenotype _ Not _ Observed

1 Reference

First Author Title Year Journal Volume Pages PubMed ID
            WBPaper00064064

0 Strain